不同被捕食细菌对新疆盐碱地粘细菌分离的影响

Influence of different prey strains on isolation of myxobacteria in saline-alkaline soils of Xinjiang

摘要:

摘要:【目的】利用被捕食细菌诱导粘细菌形成子实体的方法分离新疆盐碱地粘细菌，并初步分析被捕食菌与被诱导粘细菌之间关系。【方法】从盐碱地分离出16 株对粘细菌具有不同诱导作用的被捕食细菌，将其用以分离新疆阿克苏地区盐碱地粘细菌。【结果】对25个盐碱地土样的粘细菌进行分离，共分离到55株粘细菌，结合分子生物学手段和形态学特征，初步将其归属为粘球菌属、珊瑚球菌属、Pyxidicoccus、孢囊杆菌属和侏囊菌属及6 株无法纯化的疑似粘细菌。供试的16 株被捕食细菌诱导粘球菌属均具有较好效果，而Pyxidicoccus和孢囊杆菌只能由革兰氏阳性菌诱导。【结论】与传统分离方法比较，采用多种被捕食细菌分离粘细菌其出菌率明显提高，数量增加，且易观察和挑取，有利于后续纯化。不同粘细菌的捕食菌谱及捕食嗜好不同，增加被捕食细菌筛选范围可能会提高粘细菌的分离效率。本研究采用被捕食菌分离粘细菌的方法为粘细菌的分离提供了新思路与途径。

Abstract:

Abstract:［Objective］We isolated myxobacteria in saline-alkaline soils of Xinjiang using a prey-predator strategy where the prey bacteria can induce the predator myxobacteria to form visible fruiting body，and evaluated intrinsic relationships between prey and predator myxobacteria． ［Methods］ Sixteen bacteria with inductive effects of fruiting body formation were obtained，and then used as preys to isolate the myxobacteria． ［Results］ A total of 55 myxobacteria strains were isolated from 25 soil samples，which were identified to the genera of Myxococcus，Corallococcus，Pyxidicoccus，Cystobacter，and Nannocystis． Besides，6 unpurified isolates were believed to be myxobacteria． All the 16 prey bacteria had preferable inductive effects on Myxococcus spp． ，whereas Pyxidicoccus spp． and Cystobacter spp． were only induced by Gram-positive strains． ［Conclusion］ The prey-predator strategy provided a new and more effective way to isolate myxobacteria．