小花棘豆(Oxytropis glabra DC)内生真菌的分布特点

Distribution of endophytic fungi in Oxytropis glabra DC

摘要:

摘要:【目的】为探明小花棘豆不同组织中内生真菌带菌率及其种属分布特点，对采自内蒙古阿拉善盟草原的小花棘豆各组织内生真菌进行检测与分离。【方法】制作小花棘豆不同组织临时装片，通过染色观察与分离鉴定方法研究小花棘豆各组织中内生真菌分布形态、带菌率以及内生真菌种属分布情况。【结果】通过临时装片染色技术从小花棘豆茎、叶、叶柄及种子中均检测到内生真菌并观察到其在各组织中的分布特点;采用普通分离方法从小花棘豆四种组织中分离出79株内生真菌，鉴定为5个种。比较小花棘豆各组织内生真菌带菌率与分离率发现种子＞叶＞茎＞叶柄，且小花棘豆内生真菌的主要优势种(相对分离频率)为疯草内内生真菌(Undifilum oxytropis) (77.32%)，砖格孢属( Embellisia sp.) L12(64.00%)，镰刀菌属(Fusarium equiseti) (50.00%)。【结论】小花棘豆各组织中普遍存在内生真菌，其在各组织中的形态分布各异，内生真菌数量与种属分布存在组织差异性，种子和叶是内生真菌侵染和定殖的主要部位。

关键词:关键词:小花棘豆，内生真菌，检测，分离鉴定，分布特点

Abstract:

Abstract:［Objective］ Endophytic fungi were detected and isolated from the stems，leaves，petioles and seeds of Oxytropis glabra DC sampled from Alashan of Inner Mongolia to investigate the infection rate and species distribution in different tissues．［Methods］ The endophytic fungi infection rate and distribution of species in different tissues were investigated by making temporary slides，staining，isolation and identification．［Results］Endophytic fungi were detected and observed from all parts (stems，leaves，petioles and seeds) of the plant by temporary slides staining．A total of 79 isolates were cultivated from 4 different tissues by common separation methods，which belonged to 10 genera after identification． The infection rate and separation rate were seeds＞leaves＞stems＞petioles． Undifilum oxytropis，Embellisia sp． L12 and Fusarium equiseti were the dominant species in this plant with the relative isolate frequency 77.32%，64.00% and 50.00%，respectively． ［Conclusion］Endophytic fungi were commonly found in the all parts of Oxytropis glabra DC． There was an obviously difference in quantity，species and distribution of the endophytic fungi between different parts of plant． Seeds and leaves were the most vulnerable to infection and colonization by the endophytic fungi．

Key words:Keywords: Oxytropis glabra DC，endophytic fungi，detection，isolation and identification，distribution