肠侵袭性大肠杆菌tatABC基因缺失株的生物学特性

Biological characteristics of an enteroinvasive Escherichia coli strain with tatABC deletion

摘要:

摘要:【目的】研究双精氨酸运输系统(Tat)与肠侵袭性大肠杆菌(EIEC)生物学特性之间的关系。【方法】通过同源重组的方法，构建EIEC的tatABC基因缺失菌株以及互补菌株，并探索其对细菌形态、底物转运功能以及对HeLa细胞和豚鼠角膜侵袭力的影响。【结果】TatABC基因缺失株细菌形态变化明显，底物转运功能丧失，细菌侵袭力也显著减弱(缺失株侵入HeLa 细胞数量明显减少，致豚鼠角膜病变能力明显减弱)，而互补菌株在上述方面较接近野生株。【结论】EIEC的Tat蛋白运输系统与EIEC的生物学特性有密切关系。

关键词:关键词:肠侵袭性大肠杆菌，生物学特性，双精氨酸运输系统，毒力

Abstract:

Abstract:［Objective］ To study the relationship between twin-arginine translocation system (Tat) system with the biological characteristics of enteroinvasive Escherichia coli (EIEC)．［Methods］Through homologous recombination，we constructed EIEC's tatABC gene deletion strain and complementary strain，and explored their impact on bacterial form，substrate transport function as well as on HeLa cells and guinea pig's corneal invasion force．［Results］The tatABC gene deletion strain had apparent changes in bacterial form，loss of substrate transporter function，and significant weakened bacterial invasion force (the number of the deletion strain invading into HeLa cells was decreased significantly，and the ability of its corneal lesion capacity of the guinea pig was significantly weakened)，while the complementary strain was similar to the wild strain in the above respects．［Conclusion］EIEC's Tat protein transport system is closely related with the biological characteristics of EIEC．

Key words:Keywords: Enteroinvasive Escherichia coli，Biological characteristics，Tat protein transport system，Virulence