小单孢菌40027菌株质粒pJTU112复制区的克隆与序列特性

Cloning and sequence characterization of replication region in plasmid pJTU112 from Micoromonospora sp．40027

摘要:

摘要:福堤霉素A 产生菌———小单孢菌40027菌株含有两个质粒pJTU101和pJTU112。【目的】对质粒pJTU112复制区进行克隆，并对质粒pJTU112复制区序列进行测定和分析。【方法】克隆质粒pJTU112的不同DNA片段导入消除质粒的小单孢菌40027菌株，通过复制功能的测定，确定质粒pJTU112的复制区，并进行测序和生物信息学分析。【结果】质粒pJTU112 的复制区定位在约4.7 kb的SacI-KpnI DNA片段上，测序和生物信息学分析表明:4.7 kb的SacI-KpnI DNA片段包含5个ORFs(open reading frames)，其中pJTU112.1和pJTU112.2与质粒接合转移有关，pJTU112.3、pJTU112.4和pJTU112.5与质粒复制有关。【结论】质粒pJTU112的复制区定位在约4.7 kb的SacI-KpnI DNA片段上。

Abstract:

Abstract:Micoromonospora sp．40027，the producer of fortimicin A，harbors two plasmids pJTU101 and pJTU112．［Objective］Cloning and sequencing of replication region of pJTU112，analyzing replication region sequence of pJTU112．［Methods］Different fragments of pJTU112 were cloned and introduced by conjugation into Micromonospora sp． LXH20．The replication region of pJTU112 was identified．［Results］The replication region of pJTU112 was located in the 4.7 kb SacI-KpnI DNA fragment．DNA sequencing and analysis revealed that the 4.7 kb SacI-KpnI DNA fragment encoded five open reading frames．The pJTU112.1 and pJTU112.2 were related to plasmid conjugation，pJTU112.3，pJTU112.4 and pJTU112. 5 were related to plasmid conjugation．［Conclusion］ The replication region of pJTU112 was located in the 4.7kb SacI-KpnI DNA fragment．