微生物GH13家族淀粉脱支酶研究进展

Advances in studying microbial GH13 starch debranchingenzyme-A review

摘要:

摘要:普鲁兰酶和异淀粉酶都具有典型的(β/α)8桶状结构，属于GH13家族淀粉脱支酶。GH13家族的淀粉脱支酶能够专一、高效地水解淀粉分支部位的α-1，6-糖苷键，可以有效提高淀粉原料利用率和生产效率，在淀粉加工工业中具有重要的应用价值，因此近年来对GH13家族淀粉脱支酶的研究逐渐增多。本文系统地综述了微生物来源的GH13 家族淀粉脱支酶的国内外研究进展，分别对普鲁兰酶和异淀粉酶的底物特异性及结构基础、研究现状以及应用和研究新趋势进行了阐述。并对GH13家族淀粉脱支酶研究中存在的问题和下一步开发方向提出了见解。

Abstract:

Abstract:Pullulanase and isoamylase belong to the GH13 family (glycoside hydrolase family 13) with similar sequence，catalytic mechanism and three-dimensional fold ((β/α) 8-barrel structure)．Starch debranching enzymes can hydrolyze the α-1，6-glucosidic bonds at the branch sites of starch，and improve raw material utilization and production efficiency in the starch industry．In this review，the substrate specificity，protein structure，advances and new trends in the study of microbial GH13 starch debranching enzyme were systematically introduced．In addition，some opinions on the research status and prospect for starch debranching enzyme were discussed．