IFN-α对gp96的上调降低其抗HBV的效率

IFN-α-induced gp96 upregulation negatively affects the anti-HBV efficiency of IFN-α

摘要:

摘要:【目的】探讨IFN-α上调热休克蛋白gp96对其抗HBV作用的影响。【方法】首先通过RT-PCR、荧光报告基因和Western blot在转录和蛋白水平上研究IFN-α对gp96上调的时间、剂量依赖性。其次，利用RTPCR、ELISA分别研究转染表达gp96、RNA干扰gp96对HBV复制的影响。最后，通过ELISA、RT-PCR研究RNA干扰gp96后IFN-α抗HBV的作用。【结果】发现IFN-α对gp96的上调具有时间、剂量依赖性。而gp96的上调促进了HBV的复制，消除IFN-α对gp96的上调显著增强IFN-α抗HBV的效率。【结论】IFN-α上调gp96对其抗乙肝病毒功能起负面影响，抑制gp96上调可增强IFN-α的抗病毒效果。

Abstract:

Abstract:［Objective］To investigate the effect of the Interferon-α-induced gp96 upregulation on the anti-HBV efficiency of Interferon-α(IFN-α).［Methods］The effect of IFN-α on the transcription and expression of heat shock protein gp96 was determined by real-time PCR，luciferase reporter assay and Western blot．The effect of over-expression or knock-down of gp96 by transfection or RNA interference，and treatment with IFN-α on HBV expression and replication was examined by ELISA and RT-PCR.［Results］IFN-α treatment led to increased gp96 expression in a time- and dose-dependent manner. The overexpression of gp96 enhanced HBV expression and replication，whereas downregulation of gp96 resulted in decreased HBV replication． Finally，we verified that blocking IFN-α-induced upregulation of gp96 significantly enhanced IFN-α-mediated HBV inhibitory effects.［Conclusion］ IFN-α-induced upregulation of gp96 may negatively affect the anti-HBV function of IFN-α. These data provide valuable insight for enhancing the antiviral efficacy of IFN-α by simultaneous inhibition of gp96．