抗烟草黄瓜花叶病毒多糖筛选及其对烟草防御酶活性的影响

Polysaccharides inhibiting cucumber mosaic virus and their influence on tobacco defensive enzyme activities

摘要:

摘要:【目的】筛选出对烟草黄瓜花叶病毒病有良好抑制作用的多糖并探索其对烟叶防御酶活性的影响。【方法】采用半叶法，测定了安络小皮伞多糖等21 种真菌多糖在枯斑三生烟上对CMV 的钝化、预防及治疗效果，并测定了抗病毒多糖处理后普通烟NC-89体内防御酶的变化。【结果】安络小皮伞多糖对CMV具有较好的钝化及预防效果，其200 倍液与等量供试病毒液混合30 min后接种，钝化效果为83.41%;喷施安络小皮伞多糖200倍液24 h后接毒处理，预防效果可高达93.15%。安络小皮伞多糖对CMV 防治机理的研究 表明，多糖处理后烟草相关防御酶POD、PAL和PPO活性增强，其中喷施安络小皮伞多糖24 h后接毒处理的酶活增加最为显著，该处理烟苗的POD、PAL和PPO的酶活峰值分别可增加至对照的2.74、3.45和2.82倍。【结论】安络小皮伞多糖通过增强烟草体内防御酶活性而提高烟草对烟草黄瓜花叶病毒病的抗性。

关键词:关键词:安络小皮伞，多糖，黄瓜花叶病毒，酶活

Abstract:

Abstract:［Objective］ Polysaccharides inhibiting cucumber mosaic virus (CMV) on tobacco were screened and their influence on tobacco defensive enzyme activities was explored．［Method］We detected the deactivating，preventing and treating effect of 21polysaccharides on CMV by half leaf method on Nitcotiana tabacum var．Samsun NN． We detected the variation of enzyme activity of Nitcotianatabacum var． NC89 handled by antiviral polysaccharide．［Result］Results show that Marasmiu sandrosaceus polysaccharide had good deactivating and preventing effect on CMV． The preventing rate could reach 83. 41% when the tobacco was dealt with the admixture of its 200-fold dilution and equivalent virus liquid for 30min．The inhibition rate could reach 93. 15% when the tobacco was inoculated after spraying with M．androsaceus polysaccharide for 24h．Moreover，the diversification of enzyme activity of tobacco-related was detected． Results show that peroxidase (POD)，polyphenol oxidase (PPO) andphenylalanine ammonia lyase (PAL) activities significantly enhanced．M．androsaceus polysaccharide sprayed on tobacco after inoculating CMV for 24h．Its enzyme activities increased．The peaksof POD，PPO and PAL activities ofthe treatment were 2.74，3.45 and 2.82 times of the value of comparison，respectively.［Conclusion］M. androsaceus polysaccharide can increase the resistance of tobacco on CMV by enhancing defense enzyme activity of tobacco.

Key words:Keywords: Marasmius androsaceus，polysaccharides，cucumber mosaic virus (CMV)，enzyme activity