美洲大蠊(Periplaneta americana)肠道微生物多样性分析

Phylogenetic analysis of bacterial community in the gut of American cockroach (Periplaneta americana)

摘要:

摘要:【目的】分析美洲大蠊(Periplaneta americana)肠道微生物群落的组成。【方法】以美洲大蠊肠道微生物基因组为模板，Bact-27F和Univ-1492R为引物，PCR扩增16S rRNA基因，连接pGEM-T载体，构建肠道微生物16S rRNA基因文库，并对肠道微生物的组成及多样性进行分析。【结果】美洲大蠊肠道微生物主要包括变形杆菌门(Proteobacteria，66.4%)，拟杆菌门(Bacteroidetes，17.8%)，厚壁菌门(Firmicutes，14.5%)，梭杆菌门(Fusobacteria，0.6%)，以及未分类微生物(unclassified bacteria，0.6%)。系统发育分析显示，15%的美洲大蠊肠道微生物16S rRNA基因序列与亲缘关系较近的杂食蟑螂肠道微生物的16S rRNA基因序列聚于一支;59%的美洲大蠊肠道微生物16S rRNA基因序列与不同食性动物肠道微生物的16S rRNA基因序列聚于一支。另一方面，18%的美洲大蠊肠道微生物16S rRNA基因序列与潜在的微生物致病菌一致性高于99%，说明美洲大蠊是一类潜在的致病菌携带者。【结论】美洲大蠊肠道微生物群落组成多样，宿主系统进化以及杂食性生活方式对其肠道微生物的组成有较大影响。

Abstract:

Abstract:［Objective］ The present study was to fully evaluate the intestinal bacterial community of Periplaneta americana，an important model to study insects.［Methods］We investigated the bacterial community of P. americanagut by culture-independent methods，involving constructing the 16S rRNA gene library and microbial diversity analysis.［Results］The phylotypes were affiliated with Proteobacteria(66.4%)，Bacteroidetes (17.8%)，Firmicutes (14.5%)，Fusobacteria (0.6%) and unclassified bacteria (0.6%)．Phylogenetic analysis shows that 15% of the sequences clustered with that from a closely related omnivorous cockroach;and 59% clustered with that from more distantly related animals，including omnivorous，herbivorous，and carnivorous animals，which differ greatly in feeding habits． Moreover，18% of the clones showed high sequence identity with potential pathogens closely related to human diseases，which also reinforces the concept of the cockroach as a carrier of pathogens．［Conclusion］Due to their habits of feeding on a variety of foodstuffs，omnivorous cockroaches harbor a large and diverse microbial community in the gut． The host phylogeny and dietary habits might be critical for the intestinal bacterial community composition of cockroaches.