活的但非可培养功能菌群对多氯联苯降解的探索进展

Potential of viable but non-culturable bacteria in polychlorinated biphenyls degradation—A review

摘要:

摘要:活的但非可培养(“viable but non-culturable”，简称VBNC)状态是微生物应对各种环境压力的一种生存机制。面对日益严重的异生质污染问题，研究污染胁迫下VBNC状态菌的潜在环境功能具有重要意义。该文阐述了VBNC状态菌的研究现状，针对多氯联苯微生物降解存在的问题，重点介绍环境中潜在VBNC功能菌群的复苏培养。提出利用藤黄球菌(Micrococcus luteus)复苏促进因子(resuscitation-promoting factor，简称Rpf)探索具有潜在多氯联苯降解功能的VBNC状态菌群，为多氯联苯高效降解菌群的筛选提供新思路。同时，结合VBNC状态菌絮凝、硝化除臭等方面的探索研究，对VBNC状态菌的潜在环境功能进行了展望。

Abstract:

Abstract:The state of “viable but non-culturable”(VBNC) is a survival strategy adopted by microorganisms when exposed to environmental stress．With the increasingly serious problem of xenobiotics pollution，enhanced microbial processes that exploit the potential of microbes to remediate polychlorinated biphenyl-contaminated environments have been developed．Microorganisms represent a significant advance with respect to the transformation and degradation of polychlorinated biphenyls in the environment． It is of great importance to study the potential function of VBNC bacteria in polluted environment．In this paper，current research status of VBNC bacteria is summarized，and resuscitation of VBNC bacteria to potentially stimulate microbial degradation of pollutants is discussed in detail．Furthermore，we put forward a novel approach to explore the potential of VBNC bacteria for polychlorinated biphenyls degradation using resuscitation promoting factor (Rpf) of Micrococcus luteus．The novel efficient method is helpful for excavating and obtaining highly desirable polychlorinated biphenyls degrading microorganisms． Moreover，the prospect of VBNC bacteria to other environmental remediation fields，such as flocculation and nitrification deodorant，is addressed.