蓝细菌Synechocystis PCC 6803染色体上的基因ssl2138和sll1092构成的毒素-抗毒素系统

A hypothetical gene pair，ssl2138 and sll1092，constitutes a functional TA system on the chromosome of Synechocystis PCC 6803

摘要:

摘要:【目的】证明集胞藻(Synechocystis)PCC 6803染色体上的假定基因ssl2138和sll1092构成vapBC家族的毒素-抗毒素系统(toxin-antitoxin system，TA系统)。【方法】以RT-PCR分析ssl2138和sll1092的共转录，以选择性表达系统分析其编码产物对大肠杆菌生长的影响，并通过亲和层析和质谱检测证明编码产物之间的相互作用。【结果】ssl2138和sll1092构成的二元操纵子在正常生长条件下共转录;Sll1092表达抑制大肠杆菌的生长，Ssl2138的同时表达或随后表达可拮抗Sll1092的生长抑制作用;Ssl2138与Sll1092相互作用形成复合体。【结论】位于同一操纵子中的假定基因ssl2138和sll1092构成vapBC家族的TA系统。

Abstract:

Abstract:［Objective］We characterized the hypothetical gene pair ssl2138 and sll1092 that constitute a vapBC-family toxin-antitoxin (TA) system on the chromosome of Synechocystis PCC 6803.［Methods］An RT-PCR analysis was conducted to confirm the co-transcription of this gene pair，a selection-expression system was constructed to demonstrate the effect of their encoded proteins on E．coli growth． To validate interaction between these two proteins，an affinity capture analysis was performed．［Results］ ssl2138 and sll1092 were co-transcribed under normal growth conditions．Ectopic expression of sll1092 inhibited the growth of E．coli，which could be overcome by simultaneous or subsequent expression of ssl2138． Both proteins Ssl2138 and Sll1092 in vivo formed a protein complex due to their specific interaction．［Conclusion］ ssl2138 and sll1092 constitute a functional vapBC-family TA system of Synechocystis PCC 6803．