福州左海湖冬春季浮游细菌群落16S rRNA多样性

Diversity of bacterioplankton community during winter and spring in the Lake Zuohai in Fuzhou

摘要:

摘要:【目的】为揭示福州左海湖冬春不同季节水体中浮游细菌群落的多样性。【方法】通过对2012年1月(冬季)和2012年4月(春季)的左海湖水体样品构建细菌16S rRNA基因克隆文库，对2个季节样品中细菌群落的多样性和均匀度等进行比较，并对其群落结构特征进行分析。【结果】冬季左海细菌群落的多样性指数Shannon-Wiener(H)达到3.53，均匀度指数(E)达到0.66;春季细菌群落的多样性指数Shannon-Wiener(H)为3.37，均匀度指数(E)为0.64，这2个季节样品的细菌群落均具有很高的多样性，但均匀度都偏低。2个季节水体中共检测到5个细菌门类:α-变形菌纲(α-proteobacteria)、β-变形菌纲(β-proteobacteria)、拟杆菌门(bacteroidetes)、放线菌门(actinobacteria)、蓝细菌门(cyanobacteria)，此外还存在一些未被认知的序列。冬季的优势菌群为β-proteobacteria，次优势菌群为cyanobacteria;春季的最优势菌群为cyanobacteria。【结论】左海湖水体细菌在冬春2季节中的菌群结构存在差异，特别是最优势的细菌发生了很大变化，其中冬季细菌群落的多样性及均匀度相对较高。

Abstract:

Abstract:［Objective］To reveal the diversity of bacterioplankton during winter and spring in Zuohai lake，Fuzhou City，China．［Methods］We constructed 16S rRNA gene clone libraries in the two seasons (January，2012 and April，2012);the Shannon diversity index，Simpson dominance index and Evenness index of the bacterial community in the two samples were compared，and the analysis for the bacterial community structures of this two water samples was conducted．［Results］The Shannon diversity index in January achieved 3. 53，and the Evenness index achieved 0.66; While the Shannon diversity index of April reached 3.37，and the Evenness index reached 0.64．It indicated that the bacterial communities in the two water samples were diverse，but the Evenness indexes were very low． Bacterial belonging to 5 phyla were identified: α-proteobacteria，β-proteobacteria，bacteroidetes，actinobacteria and cyanobacteria．Also，there were many sequences belonging to unidentified bacteria．In January， bacterial community with β-proteobacteria predominated followed by cyanobacteria; while cyanobacteria was the predominant bacterial communities in April.［Conclusion］The distribution of the bacteria in the two seasons has differences，especially the most dominant bacteria differed significantly．In addition，bacterial diversity in the two water samples was high，and compared with in spring，that in winter has higher bacterial diversity，but exit a large number of unidentified bacteria in this environment.