革兰氏阴性细菌β-桶状结构外膜蛋白折叠与组装的研究进展

Research progress on the folding and membrane-insertion mechanisms of β-barrel outer membrane protein of gramnegative bacteria-A review

摘要:

摘要:β-桶状结构外膜蛋白是革兰氏阴性细菌细胞外膜层的主要组成部分，在营养吸收、维持外膜完整性、病原菌致病性及多重耐药性等方面发挥着重要的作用，对细菌的存活至关重要。详细了解这些蛋白的合成、折叠与组装到外膜的过程在增加筛选抗病原菌药物靶位、增强有益菌的生物活性等方面具有重要意义。本文对近年来革兰氏阴性细菌β-桶状结构外膜蛋白的合成、转运、折叠及组装到外膜过程的研究结果进行了综合论述，重点叙述了β-桶状结构外膜蛋白组装复合体的研究进展，并在此基础对这一类蛋白的折叠与膜整合过程提出一些新的见解，便于读者快速、全面了解该领域的最新发现和发展。

Abstract:

Abstract:β-Barrel outer membrane proteins are the major components of the outer membrane of Gram-negative bacteria，which are in contact with the extracellular environment directly．β-barrel outer membrane proteins play key roles in nutrients absorption and keeping membrane integrity．They are also involved in the pathogenicity and multiple-antibiotic resistance of pathogenic bacteria． Therefore，β-barrel outer membrane proteins are very important for the survive of bacteria cells，and full understanding of the biosynthesis，folding and membrane insertion of these proteins is of great significance for fighting against pathogenic bacteria and utilization of beneficial bacteria． In this article，the research progress on the biosynthesis in cytoplasm，the translocation across the inner membrane，transportation to periplasm and the folding and membrane insertion of β-barrel outer membrane proteins are reviewed，and the progress on the study of OMPs assembly machinery is emphasized．