分枝杆菌glpX基因的功能

Gene function of glpX in mycobacterium

摘要:

摘要:【目的】构建耻垢分枝杆菌(Mycobacterium smegmatis)glpX基因敲除株，研究其在生理代谢中的功能。【方法】利用分枝杆菌噬菌体Che9c重组系统构建耻垢分枝杆菌glpX 基因敲除株;比较野生株及突变株在不同碳源培养条件下的生长差异;通过荧光实时定量PCR，比较野生株在以葡萄糖或油酸为唯一碳源培养下，glpX基因的表达水平。【结果】glpX突变株在以甘油或油酸为唯一碳源的培养基中无法生长;野生株在以油酸为唯一碳源培养下，glpX基因表达上调。【结论】glpX基因编码了分枝杆菌糖异生途径必需的和非冗余的果糖1，6-二磷酸酶(fructose 1，6-bisphosphatase，FBPase)。

Abstract:

Abstract:［Objective］To investigate the function of glpX gene in mycobacterial physiology and metabolism.［Methods］The glpX mutant of Mycobacterium smegmatis was constructed byusing mycobacteriophage Che9c-encoded recombination system．Then the growth difference between mutant and wild type strain was compared on various carbon sources．Furthermore，we analyzed the glpX gene transcriptional level of wild type strain growing on glucose or oleic acid by realtime PCR．［Results］The growth of M．smegmatis on glycerol or oleic acid is strongly attenuated after the deletion of the glpX gene．The transcription of glpX gene is upregulated in the medium with oleic acid as the sole carbon source． ［Conclusion］ These results indicate that the fructose 1，6-bisphosphatase encoded by glpX gene is required and nonredundant for mycobacterial gluconeogenesis.