南海局部海洋沉积物中真菌多样性及产酶活性

Fungal diversity and enzyme activities in marine sediments in the South China Sea

摘要:

摘要: 【目的】从11份南海海洋沉积物中分离耐盐真菌，并对其物种多样性及产酶活性进行研究。【方法】利用平板涂布法分离耐盐真菌，基于形态学和ITS 序列的系统进化研究耐盐真菌多样性; 利用6种筛选培养基对耐盐真菌进行产酶活性筛选。【结果】分离得到1689株耐盐真菌，共41个形态种。形态学和ITS序列分析表明，这些真菌归于15 个属，其中曲霉属(Aspergillus)和青霉属(Penicillium)为优势菌群。对已测序的41株耐盐真菌的产酶活性研究表明，8株产纤维素酶，9株产淀粉酶，5株产复合酶，1 6株产蛋白酶，3株产脂肪酶，未发现产壳聚糖酶的菌株，其中Acrodontium sp. 8m和Aspergillus sp. 86b产复合酶的活性相对较高，而Penicillium sp. 41m产蛋白酶的活性相对较高。【结论】南海局部海洋沉积物中耐盐真菌丰富，多数菌株具有产酶活性。

Abstract:

Abstract: ［Objective］We studied species diversity and enzyme activities of fungi from 11 marine sediment samples in the South China Sea.［Methods］ Salt-tolerant fungi were isolated by a dilution-plate method，and their diversity was studied based on fungal morphology and rDNA ITS (Internal Transcribed Spacer) sequences. Enzyme activities were screened by six special selective media.［Ｒesults］ A total of 1689 strains of salt-tolerant fungi were isolated．Morphology and ITS sequence analysis identified these fungi to 41 species of 15 described genera，of which Aspergillus sp．and Penicillium sp．were the dominant populations． Studies on enzyme activities of 41 sequenced strains showed that 8 strains produce cellulase，9 strains produce amylase，5 strains produce compound enzyme，16 strains produce protease，3 strains produce lipase and no strain produce chitonsanase．Acrodontium sp．8m and Aspergillus sp．86b produced the most multiple enzymes，while Penicillium sp．41m produced comparatively higher protease．［Conclusion］ There were abundant salttolerant fungi from marine sediment samples in the South China Sea，and more strains had enzyme activities.