猪链球菌1、2、14、1/2型荚膜多糖的单糖组成比较

Comparison of monosaccharide composition of capsular polysaccharides in Streptococcus suis serotype 1，2，14 and 1/2

摘要:

摘要:【目的】猪链球菌1、2、14和1/2型间存在单向或双向的交叉抗原性，这种交叉抗原性的产生原因至今未被揭示。【方法】采用Sephacryl S-300凝胶层析柱对猪链球菌14 和1/2型荚膜多糖进行分离纯化，经苯酚-硫酸检测和dot-ELISA辅助鉴定，确定荚膜多糖成分。采用高效凝胶渗透色谱法测定14和1/2型猪链球菌荚膜多糖分子量分别为487.38 kDa和512.72 kDa。【结果】经柱前衍生高效液相色谱法、荧光标记液相色谱法和核磁共振测定14和1/2型猪链球菌荚膜多糖单糖组成分别为:Glc/Gal/GlcNAc/Rha/Neu5Ac(1:2. 94:1.35:0.24:0.37)和Glc/Gal/GlcNAc/GalNAc/Rha/Neu5Ac(1:1.67:1.05:0.93:0.72:0.7)。并与已知的猪链球菌1、2型荚膜多糖的单糖组成进行比较分析，发现4种血清型荚膜多糖都具有Glc、GlcNAc、Gal和Neu5Ac，但单糖组成和比列并无明显相似性，这种交叉抗原性可能是由于荚膜多糖的空间结构相似性和(或)细胞表面的其他成分引起的。

关键词:关键词:猪链球菌，血清型，荚膜多糖，单糖组成

Abstract:

Abstract:［Objective］There are one-way or two-way cross-reactions among Streptococcus suis serotype 1，2，1/2 and 14，the reason to which was unknown． ［Methods］ The capsular polysaccharides of serotype 14 and 1/2 were purified on Sephacryl S-300 column and identified by phenol-sulphuric acid method and dot-ELISA．The molecular weight of the serotype 14 and 1/2 capsular polysaccharides was revealed as 487.38 kDa and 512. 72 kDa by high performance gel permeation chromatography，respectively.［Results］The monosaccharide composition of serotype 14 and 1/2 capsular polysaccharides was determined as Glc/Gal/GlcNAc/Rha/Neu5Ac (1:2.94:1.35:0.24:0.37) and Glc/Gal/GlcNAc/GalNAc/Rha/Neu5Ac (1:1.67:1.05:0.93:0.72:0.7)by pre-column derivatization high performance liquid chromatography，fluorescent labeling HPLC and NMR，respectively．These were compared with the composition of serotype 1 and 2 capsular polysaccharides．Glc，GlcN，Gal and Neu5Ac was contained in the capsular polysaccharides of serotype 1，2 14 and 1/2．But there is no prominent correlation between the monosaccharide composition and crossreactions．The cross-reactions among them could be induced by the structure of the capsular polysaccharides and/or the other components on the cell wall．

Key words:Keywords: Streptococcus suis，serotype，capsular polysaccharide，monosaccharide composition