新城疫病毒非编码序列的遗传演化趋势

Evolution of non-coding nucleotide sequences in Newcastle disease virus genomes

摘要:

摘要:【目的】探讨新城疫病毒全基因序列中非编码序列的分子演化规律。【方法】结合本研究室2012年自产蛋下降鸭群中分离测序的一株鸭源新城疫病毒全序列，从GenBank 下载35株不同基因型新城疫病毒全长cDNA序列，获取非编码序列，分别绘制引导序列、尾随序列、F-HN及HN-L基因间隔序列(IGS)的遗传进化树，比较编码基因内5’及3’UTR 序列核苷酸序列替代特点。【结果】非编码序列的长度及位置高度保守，而其核苷酸基因序列在不断发生变异，且变异趋势与编码基因序列相一致。【结论】新城疫病毒在整个基因组上编码和非编码序列同步发生变异。

Abstract:

Abstract:［Objective］Although much is done in the coding genes of Newcastle disease virus (NDV)，limited papers can be found with non-coding sequences．In this paper，the evolution tendency of non-coding sequences was studied．［Methods］NDV strain LC12 isolated from duck with egg drop syndrome in 2012，and others 35 strains genome cDNA of different NDV genotype were sought and obtained from GenBank． Analytical approaches including nucleotide homology，nucleotide alignment and phylogenetic tree were associated with the leading sequences，trailer sequences，intergenic sequences (IGS)，and coding gene between 5 ’and 3’UTＲ nucleotide，respectively．［Results］The location and the length of the non-coding sequences highly conserve，and the variation trend of non-coding sequences is synchronous with the entire genomes and coding genes． ［Conclusion］The molecular variation of the coding gene was indistinguishable with the non-coding gene in view of the NDV genome.