乳酸乳球菌KLDS4.0325的糖代谢过程及乳酸生物合成途径的比较

Carbohydrate metabolism and lactic acid biosynthesis of Lactococcus lactis subsp．lactis KLDS4.0325

摘要:

摘要:【目的】为了探究乳酸乳球菌乳酸亚种KLDS4.0325的碳水化合物利用能力和乳酸形成潜力。【方法】本文对该菌株进行了全基因组鸟枪法测序，并应用生物信息学方法对该菌株细胞外糖的转运、代谢及产酸途径涉及的一系列基因与其它9 株参考菌株进行了比较分析。【结果】与参考菌株相比，该菌株基因组中具有较多涉及整个途径糖代谢途径的关键酶编码基因。【结论】该菌株在基因水平上表现出能够利用多种糖类物质来产乳酸的优良性状，是一株具有高产L-乳酸工业潜能的乳酸菌。

Abstract:

Abstract:［Objective］We aimed to study the carbohydrate metabolism and lactic acid biosynthesis of Lactococcus lactis KLDS4.0325．［Methods］Whole genome shot gun strategy was used for genome sequencing of strain L．lactis KLDS 4.0325．Then，using bioinformatics method，we compared a series of protein－coding genes involved in transporting extracellular carbohydrate，sugar metabolism and lactic acid biosynthesis of strain L． lactis KLDS4．0325 with other 9 reference strains．［Results］In L．lactis KLDS4．0325 genome，where possesses more key enzyme coding genes related to the whole pathway of sugar metabolism than reference strains．［Conclusion〗In gene level，therefore，strain L．lactis KLDS4．0325 shows a remarkable characteristic by utilizing various sugar to produce lactic acid，is a lactic acid bacteria with industrial potential of high yield L－lactic acid．