植物乳杆菌ZJ8吸附伏马菌素B1和B2特性

Absorption of fumonisin B1 and B2 by Lactobacillus plantarum ZJ8

摘要:

摘要:【目的】研究了植物乳杆菌(Lactobacillus plantarum) ZJ8对伏马菌素B1和B2吸附作用与机制。【方法】采用高效液相色谱检测菌体对FB1和FB2的吸附率。【结果】菌株ZJ8对FB1和FB2的吸附率分别为89.9%、95.0%，这种吸附特性与菌体活力无关，且随培养时间延长而增加。菌株ZJ8的吸附率在pH 4时达到最大，分别为96.4%和99.0%。碱性和高温条件下都不利于菌株吸附伏马菌素。经强酸和SDS处理后，菌体对伏马菌素吸附率显著性上升。菌体细胞壁对FB1和FB2的吸附率高达96.8%和100%。植物乳杆菌 ZJ8胞壁成分中肽聚糖的吸附率最高，分别为98.4%和100%。【结论】植物乳杆菌ZJ8可以通过吸附作用清除环境中的伏马菌素B1和B2，对吸附起主要作用的是菌体细胞壁上的肽聚糖。

Abstract:

Abstract:［Objective］ We studied the ability of Lactobacillus plantarum ZJ8 to bind fumonisins FB1 and FB2．［Methods］The percentage of FB1 and FB2 bound by the strain was measured by HPLC after bacterial cells and FB1 and FB2 were co-incubated in MＲS media at 37℃ for 4 h.［Results］The percentage of FB1- and FB2 -binding was 89.9% and 95.0%，respectively．The FBs-binding rate of strain ZJ8 reached the maximum at pH 4．Binding of FBs was poor under alkaline conditions and high temperatures．After acid and SDS treatment，the FBs-binding rate significantly increased．The percentage of FB1- and FB2 -binding was 96.8% and 100% for the cell walls of strain ZJ8，respectively．Moreover，about 96.8% FB1 and 100% FB2 was bound to the peptidoglycan of strain ZJ8’s cell walls.［Conclusions］L．plantarum ZJ8 has potential to remove fumonisins．The peptidoglycan of cell walls from strain ZJ8 was proved to be the main site of FBs-binding．