双歧杆菌增强小鼠机体的免疫功能

Effect of Bifidobacterium on the immunity in BALB/c mice

摘要:

摘要:【目的】本研究针对实验室自行从内蒙传统发酵乳制品中分离得到的两株双歧杆菌Bifidobacterium adolescentis BB-2和B.longum BB-3，对其调节机体免疫的功能进行了评价。【方法】采用健康SPF级BALB/c小鼠，每组10只，空白组灌胃无菌脱脂乳，阳性对照组灌胃含有商业菌株BB-12的无菌脱脂乳，处理组同样以无菌脱脂乳为溶液，灌胃含有不同剂量的Bifidobacterium adolescenti BB-2或B.longum BB-3，测定细胞免疫(迟发型变态反应DTH、脾淋巴细胞增殖MTT 显色反应、自然杀伤细胞活性)，体液免疫(绵羊红细胞免疫后的血清溶血素活性)，以及非特异性免疫(巨噬细胞吞噬活性)指标。【结果】表明BB-2和BB-3两株双歧杆菌均能够增强DTH反应。双歧杆菌组小鼠的巨噬细胞的吞噬活性也有提高，同时自然杀伤细胞活性及血清溶血素水平也高于对照组，菌株处理组的脾淋巴细胞增殖率也有提高。剂量效应不显著，其中低剂量浓度(102－6 cfu/mL)即可发挥作用。【结论】证明双歧杆菌BB-2和BB-3能够促进小鼠先天性免疫力和获得性免疫力的提高。本研究的开展对开发我国具有自主产权的功能菌株具有重要意义。

关键词:关键词:青春双歧杆菌，长双歧杆菌，细胞免疫，体液免疫，免疫促进

Abstract:

Abstract:［Objective］ We studied the system immunofuctions of two Bifidobacterium strains isolated from food．［Methods］There were 10 SPF BALB /c mice in each group．The control group was given only sterile skim milk．The positive control group was given sterile skim milk containing commercial strain BB-12．The treatment group was given sterile skim milk containing different dosages of B．adolescentis BB-2 or B．longum BB-3．The immune parameters including cellular immunity (delayed-type hypersensitivity ［DTH］，splenic lymphocyte proliferation and natural killer ［NK］ cell activity)，humoral immunity (serum hemolytic activity in immunized animals)，and nonspecific immunity (peritoneal macrophages phagocytsis) were measured．［Results］Ingestion of B．adolescentis BB-2 or B．longum BB-3 could increase the DTH response． Macrophage phagocytsis was also enhanced，while activities of the NK cells and levels of the serum hemolysin were also significantly higher than that in the control group． There was a significant increase in splenic lymphocyte proliferation in bifidobacteria treated mice compared to the control．［Conclusion］ Ingestion of B．adolescentis BB-2 or B．longum BB-3 could enhance both innate and acquired immunity in healthy BALB/c mice．

Key words:Keywords: Bifidobacterium adolescentis，Bifidobacterium longum，Cellular immunity，Humoral immunity，Immune enhancement