乌梁素海富营养化湖泊湖滨湿地过渡带细菌群落结构的高通量分析

High-throughput analysis of bacterial community of transition zone in littoral wetland of Wuliangsuhai eutrophic lake

摘要:

摘要:【目的】揭示乌梁素海富营养化湖泊湖滨湿地沉积物与土壤过渡带细菌群落的组成、丰度以及多样性变化，结合土壤理化因子探讨其对细菌群落结构的影响。【方法】采用湿地土壤总DNA提取方法提取沉积物和土壤总DNA，对细菌群落的16S rRNA 基因的V1-V3区进行高通量测序，分析各样品中细菌群落结构的组成、丰度以及多样性指标;土壤理化性质采用标准方法测定，分析其对细菌群落结构的驱动作用。【结果】富营养化湖泊湖滨湿地水陆过渡带的芦苇沼泽沉积物、碱蓬群落盐碱化土壤和白刺群落荒漠化土壤中，细菌群落组成和各类群细菌的相对丰度差异较大，门水平上的细菌类群主要有Proteobacteria、Bacteroidetes、Chloroflexi、Actinobacteria、Planctomycetes和Gemmatimonadetes，细菌群落多样性指数随陆向分布依次在增高，门水平上Proteobacteria和属水平上Sulfurimonas对湖泊退化演化敏感;环境因子最佳子集为总磷、水溶盐总量和铵态氮的组合对整个细菌群落结构的影响最为明显，相关系数最高(R=0.8857)，Mantel检验结果表明这种相关关系为显著相关(P=0.037)。【结论】乌梁素海富营养化湖泊湖滨湿地过渡带细菌群落结构差异较大，Sulfurimonas属在乌梁素海富营养化湖泊沉积物的生物地球化学循环中扮演着重要的角色，应在以后的研究中得到更多的关注。

关键词:关键词:富营养化湖泊，湖滨湿地，沉积物，细菌群落结构，空间异质性

Abstract:

Abstract:［Objective］We studied soil bacterial community composition，abundance and diversity of transition zone along eutrophic lakeside wetland sediments and soils．［Methods］The total DNA was extracted according to the sediment DNA extraction．Then high-throughput pyrosequencing was used to detect soil bacterial community composition，abundance and diversity based-on 16S rRNA gene．Soil physicochemical properties were tested to analyze its effects on bacterial community according to standard methods．［Results］The soil bacterial community composition and relative abundance were very different across transition zone in littoral wetland．Bacteria groups mainly include Proteobacteria，Bacteroidetes，Chloroflexi，Actinobacteria，Planctomycetes and Gemmatimonadetes at phylum level．The diversity index of bacterial communities gradually increased according the land distribution，especially the phylum Proteobacteria and the genus Sulfurimonas． Correlation analysis indicated that the combination of total phosphorus，total water soluble salt and ammonium has the most significant effects on the whole bacterial community structure，and Mantel Test results indicated that the correlation was statistically significant (R=0.8857，P=0.037)．［Conclusion］ The bacterial community structure of transition zone is quite different in littoral wetland of Wuliangsuhai eutrophic lake，where Sulfurimonas play potential important roles in biogeochemical cycles of sediments in Wuliangsuhai Lake.

Key words:Keywords: Eutrophic Lake，Lakeside wetlands，Sediments，Bacterial community structure，Spatial heterogeneity