肇庆星湖湿地放线菌多样性

Actinobacterial diversity in Xinghu Wetland

摘要:

摘要:【目的】了解肇庆星湖湿地放线菌多样性及其与底泥理化性质的关系。【方法】应用聚合酶链式反应-变性梯度凝胶电泳(polymerase chain reaction-density gradient gel electrophoresis，PCR-DGGE)技术对星湖湿地10个样点放线菌群落进行研究，根据DGGE图谱及克隆测序结果对其物种多样性进行分析，并结合样点理 化性质对其空间分布特征进行分析。【结果】通过PCR-DGGE指纹图谱发现各样品放线菌物种丰富度(S)、多样性指数(H')和均匀度指数(J')均有所不同。对不同样点放线菌群落相似性进行比较，发现它们的相似性系数存在着一定的关系，相邻样点间相似性较高。对DGGE优势条带进行回收、克隆、测序，显示优势类群主要分布于类诺卡氏菌科( Nocardioidaceae)、链霉菌科(Streptomycetaceae)、小单孢菌科(Micromonosporaceae)、微球菌科(Micrococaceae)、纤维素单胞菌科(Cellulomonadaceae)和原小单胞菌科(Promicromonosporaceae)。典型对应分析结果表明，星湖湿地放线菌群落结构变化的主要影响因子为底泥水溶性碳(WSOC)和速效磷(A-P)。【结论】肇庆星湖湿地蕴含着大量的放线菌资源，具有较高的物种多样性，需要进一步挖掘这些新资源。

关键词:关键词:聚合酶链式反应-变性梯度凝胶电泳，放线菌群落，典型对应分析

Abstract:

Abstract:［Objective］ The purpose of this study was to examine the relationship between the microbial community structure of soil actinomycetes and geochemical characteristics in Xinghu wetland sediments.［Methods］ The diversity and composition of the actiniobacterial community in sediments collected from 10 locations were studied using PCＲdenaturing gradient gel electrophoresis．Canonical correspondence analysis was carried out to examine the relationship between environmental variables and actinobacterial community composition．［Result］There were significant differences among the Shannon-wiener index，Ｒichness and Evenness．Similarity analysis of actinobacteria communities in different station showed certain trends in similarity coefficients，and actinobacteria community structure similarity of the same transect was much higher than others．According to the results of sequence analysis of DGGE dominant bands belonged to the orders Nocardioidaceae， Streptomycetaceae， Micromonosporaceae， Micrococaceae， Cellulomonadaceae and Promicromonosporaceae．Canonical correspondence analysis showed that sediment water-soluble organic carbon and available phosphorus were identified as the key factor in regulating the variations of actinobacterial community composition．［Conclusion］ Xinghu Wetland is the potential place for actinobacteria diversity．This study provides fundamental data for further studies of the underline mechanisms of structure microbial groups and the isolation of actinobacteria strains of interest．

Key words:Keywords: polymerase chain reaction density-gradient gel electrophoresis (PCR-DGGE)，actinobacteria community structure，Canonical correspondence analysis