Abstract:[Objective]To study the diversity of ectomycorrhizal fungi associated with Picea asperata in Xin Jiashan Forest of Qinling Mountains.[Methods]This method combined the field investigation,morphological and molecular biology to identify ectomycorrhizal fungi. [Results]There were 37 different ectomycorrhizal fungi under 14 genera of 10 families on spruce in Xin Jiashan Forest of Qinling Mountains,34 types belonged to Basidiomycetes,1 to Ascomycete and 2 to unknown species. Among these identified ectomycorrhizal fungi types,Inocybe sp. was the dominant group; Russula nauseosa was the most dominant species; Hygrophorus sp.,Tomentella coerulea,Inocybe sp.1,Helotiaceae sp. and Lactarius deterrimus were common species; and the rest species were rare species.[Conclusion]The large number but relatively rare species of dominant family and the small number but relatively more species of rare family survived in ectomycorrhizal fungal communities of Picea asperata. For the extreme degradation in arid area of western ecological system,identifing some rare family for further development and utilization had very important practical significance.