氟化物对家蚕肠道微生物菌群的影响

Effect of fluoride on gut microflora of silkworm (Bombyx mori)

摘要:

摘要:【目的】探讨氟化物对家蚕肠道微生物菌群的影响，开发具有潜在应用价值的益生菌以提高家蚕对氟化物的抗性。【方法】PCR扩增家蚕肠道内细菌16S rRNA基因并构建克隆文库;用核糖体DNA限制性分析(Amplified ribosomal DNA restriction analysis，ARDRA)方法对克隆子进行分型。从家蚕T6和734肠道样品中共获得14种分类操作单元(Operational taxonomic unit，OTUs)，以16S rRNA基因为基础构建系统发育树进行分析。再经巢式PCR-DGGE技术检测家蚕肠道内优势菌群的变化。【结果】结果表明:家蚕氟中毒后肠道内肠球菌属Enterococcus和芽孢杆菌属Bacillus细菌菌群减少，而葡萄球菌属Staphylococcus的细菌增多。【结论】氟化物能通过改变家蚕肠道内细菌的多样性和比例，从而破坏家蚕肠道微生物菌群平衡，且对家蚕734的影响作用大于T6。

Abstract:

Abstract:［Objective］ We examined the effect of fluoride on gut microflora of silkworm.［Methods］ After DNA extraction and PCR amplification，clone libraries of 16S rRNA gene fragment were constructed． Amplified ribosomal DNA restriction analysis (ARDRA) was performed by digestion of the 16S rRNA gene，and each unique restriction fragment polymorphism pattern was designated as an operational taxonomic unit (OTU)．A total of 14 OTUs were identified from intestinal samples of both T6 and 734. Phylogenetic trees of bacterial 16S rRNA nucleotide sequences were constructed and analyzed． Furthermore，the dominant bacteria were studied by the nested polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DDGE) technology.［Results］After fluorosis，the flora of Enterococcus and Bacillus reduced． However，the flora of Staphylococcus increased.［Conclusion］Fluoride can destroy the balance of microflora in the gut of silkworm by changing the bacteria diversity and proportion，which has bigger effect to 734 than T6.