PTTG1通过P53促进HBV的复制

PTTG1 enhances Hepatitis B Virus replication through P53

摘要:

摘要:【目的】探究垂体瘤转化基因1对乙型肝炎病毒复制的影响。【方法】通过酶联免疫吸附反应、实时定量PCR、双荧光报告系统检测、免疫印迹分析，研究垂体瘤转化基因1对乙型肝炎病毒的复制影响及其机制。【结果】发现垂体瘤转化基因1 促进乙型肝炎病毒复制，是通过降低P53的水平，削弱P53对HBV增强子I和II的抑制作用实现的。【结论】垂体瘤转化基因1通过抑制P53，促进乙型肝炎病毒的复制。

Abstract:

Abstract:［Objective］To study the effect of pituitary tumor-transforming gene 1 (PTTG1) on Hepatitis B Virus (HBV) replication.［Methods］ The effect and mechanism of PTTG1 on HBV replication were examined by enzyme-linked immunosorbent assay (ELISA)，immunoblot analysis，real-time PCR，dual-luciferase reporter assays and immunoblot analysis.［Results］PTTG1 impairs the repression of P53 on HBV enhancer I and enhancer II through decreasing the protein level of P53，resulting in promotion of HBV replication.［Conclusion］PTTG1 enhances HBV replication through suppression of P53.