青枯菌Rsc1285参与III型分泌系统及致病力的调控

Regulation of rsc1285 gene in type III secretion system in Ralstonia solanacearum

摘要:

摘要:【目的】研究青枯菌Rsc1285参与调控其III型分泌系统(Type III secretion system，T3SS)及致病力的途径。【方法】通过基因敲除、基因互补等研究Rsc1285对T3SS基因表达和致病力的影响。【结果】青枯菌rsc1285基因缺失突变体对寄主西红柿植株的致病力明显减弱，其hrpB、T3SS等基因表达水平较野生型明显降低，但hrpG、prhG的表达不受影响。【结论】青枯菌通过一个全新的途径利用Rsc1285调控hrpB及T3SS的转录表达并决定其致病力。

关键词:关键词:青枯菌，III型分泌系统，hrp基因簇，致病力

Abstract:

Abstract:［Objective］ Rsc1285 is one of the putative T3SS-regulated factors in Ralstonia solanacearum，and the regulation of Rsc1285 on T3SS and pathogenicity was characterized.［Methods］ The rsc1285 deletion mutants were constructed by homologous recombination and characterized by complementation.［Results］ The rsc1285 mutant was significantly less virulent than the wild-type strain to infect tomato plants. Rsc1285 controls the expression of hrpB and HrpB-regulating genes，but it is dispensable for the expression of hrpG and prhG.［Conclusion］R.solanacearum uses Rsc1285 to control the T3SS and pathogenicity via a novel pathway，and this finding provides insights into overall infection mode of R.solanacearum.

Key words:Keywords: Ralstonia solanacearum，Type III secretion system，hrp regulon，pathogenicity