福州左海湖细菌群落与环境的关系

Relationship between bacterial community and its environmental in Zuohai Lake，China

摘要:

摘要:【目的】揭示福州左海湖水体细菌群落时空动态变化与其相应环境因子的关系。【方法】通过DAPI荧光染色法、变性梯度凝胶电泳(DGGE)和构建16S rRNA基因克隆文库分析相结合的分子生物学技术，系统研究了福州左海湖在2011－2013为期两年的水体细菌丰度与群落结构的时空变化情况，并结合多元统计分析的方法，探讨了细菌群落组成与相关环境因子的对应关系。【结果】左海湖全年总细菌介于4.91×106－18.71×106 cells/mL之间，细菌丰度具有显著的季节性变化特征;DGGE和克隆文库分析结果表 明，左海湖细菌群落结构空间差异不明显，季节变化对菌群结构的影响更为显著;系统进化分析表明，左海湖细菌菌群归属于11个门类，其中最优势菌群为β-Proteobacteria和Cyanobacteria，较优势菌群为α-Proteobacteria、Actinobacteria 和Bacteroidetes。冗余分析(RDA)结果显示，pH、水温(WT)、总氮(TN)、总磷 (TP)和叶绿素a(Chla)是影响左海湖细菌群落组成的主要环境因子。【结论】左海湖中存在较丰富的细菌多样性，水体环境的季节间差异对其细菌群落组成的影响要大于不同采样点间的差异，但在优势菌群组成方面比较稳定，均为β-Proteobacteria和Cyanobacteria。

Abstract:

Abstract:［Objective］To reveal the relationship between bacterial community and the environmental in Zuohai lake，Fuzhou City．［Methods］The abundance of total bacteria was studied using DAPI staining; the composition，distribution and the dynamics of bacterial community were examined using both the denaturing gradient gel electrophoresis (DGGE) and the 16S rRNA gene clone library analyses over a 2-year periods from October 2011 to August 2013． Moreover，multivariate statistical analysis was used to analyze the relationship between bacterial community and environmental factors．［Results］The total numbers of bacteria ranging from 4.91×106 cells/mL to 18.71×106 cells/mL，and the seasonal variation of bacterial abundance was obviously stronger than the spatial heterogeneity． DGGE and clone library analysis revealed that bacterial communities were mainly affected by temporal changes． Phylogenetic analysis showed that bacteria belonging to 11 phyla were identified． Members of β-Proteobacteria and Cyanobacteria group were the predominated lineage，followed by α-Proteobacteria，Actinobacteria and Bacteroidetes． Redundancy analysis (RDA)suggested that water temperature，pH，total nitrogen，total phosphorus and chlorophyll a content contributed significantly to the bacteria-environment relationship．［Conclusion］There is rich bacterial diversity in Zuohai lake，the patterns of change in bacterial communities showed that the seasonal difference might have a significant impact on structuring the bacterial communities in the lake.