附着材料和盐度对静置培养条件下双眉藻生长和附着强度的影响

Effects of salinity and adhesive materials on the growth and adhesion of Amphora sp． HN08 under static conditions

摘要:

摘要:【目的】对1 株热带底栖硅藻———双眉藻(Amphora sp. HN08)的盐度和附着材料等培养条件进行优化。【方法】分析测定双眉藻在琼脂板、玻璃、PVC塑料板、塑料保鲜膜和尼龙网5种附着材料和6个盐度梯度(10‰–60‰)下处理9 d后的生物质产量、光合色素含量和细胞附着状态。【结果】双眉藻在玻璃板和PVC塑料板上的生物质产量最高，细胞干重达到3.64 g/m2;细胞在玻璃上的附着强度为III级，容易刮取和采用离心法脱水。盐度对双眉藻生物质产量无极显著影响，但对其附着性和光合色素含量有显著影响。盐度为30‰和40‰的培养液中，细胞的附着强度为IV级，易于离心采收;在50‰和60‰高盐度条件下的藻细胞多悬浮于培养基中，离心法较难采收。盐度大于30‰培养条件下的双眉藻类胡萝卜素含量极显著高于低盐条件下(10‰和20‰)，50‰盐度条件下的Chl a和类胡萝卜素含量可达干细胞重的26.27%和11.11%。【结论】从采收性和生物质量两方面考虑，双眉藻的最佳生长和采收培养条件为:附着材料选用玻璃板，盐度为30‰–40‰;然而综合安全性和成本考虑，建议将来生产上采用PVC塑料板作为附着材料。

Abstract:

Abstract:［Objective］To optimize the cultural conditions including adhesive materials and salinity for one tropical benthic diatom (Amphora sp． HN08)．［Methods］Two experiments were performed: (1) five adhesive materials including agar，glass，PVC plate，plastic film and nylon net were used to culture Amphora sp． HN08 HN08; (2) Amphora sp． HN08 was cultured under 6 salinity levels from 1 through 6%．The algal cells were harvested after 9 days treatments，and the biomass productivity，adhesive strength and pigments content of cells were examined.［Results］ For the adhesive materials experiment，cultures with glass and plastic plate showed the highest biomass production and the dry weight of cells reached to 3.64 g/m2．The adhesion strength level of cells on the glass plate was III degree，which means the cells were easy to be separated from the glass and dewatered by centrifugation． Salinity did not contribute significantly to the biomass production，while it significantly influenced the cells adhesion and pigments concentration． The adhesive strength levels of the cells under 3% or 4% salinity was IV degree，which led to an easy harvest process． While the cells cultured under high salinity (5% and 6% ) usually suspended in the medium and were difficult to be dewatered by centrifugation． Both chlorophyll a and carotenoid content of cells cultured with higher salinity (≥3%) are extremely higher (p＜0.01) than that with lower salinity (1% and 2%)．Chlorophyll a and carotenoid content of cells under 5% salinity was 26. 27% and 11. 11% by dry weight，respectively．［Conclusion］Taking the harvest and biomass production together，we think the optimal salinity for Amphora sp． HN08 was between 3% and 4%，and the glass plate was suitable for adhesive material．However，considering the cost and safety，the PVC plate is suggested to be used for biomass production．