烟草黑胫病菌的表型组学分析

Phenotypic analysis of Phytophthora parasitica by using high throughput phenotypic microarray

摘要:

摘要:【目的】为了阐明烟草黑胫病菌的代谢表型特征。【方法】采用BIOLOG细胞表型芯片技术系统地研究了烟草黑胫病菌的细胞表型;采用PM1－10代谢板，对烟草黑胫病菌的950种代谢表型进行了测定。【结果】黑胫病菌能代谢74%的碳源、96%的氮源、100%的硫源和98%的磷源;高效代谢的碳源为有机酸类和碳水化合物类，高效代谢的氮源为氨基酸类;病原菌具有285种不同的氮源代谢通路;黑胫病菌具有广泛的适应性，能在高达1%氯化钠、3%氯化钾、5%硫酸钠、20%乙二醇、2%甲酸钠、5%尿素或2%乳酸钠中存 活;其适应pH 值范围为3.5－10，最适7.0;在多种氨基酸的作用下，烟草黑胫病菌均表现出脱羧酶和脱氨酶活性。【结论】这些代谢特征为烟草黑胫病菌进一步的生理和代谢研究提供了理论基础，并对烟草黑胫病的防治提供了新的思路。

关键词:关键词:烟草黑胫病，Biolog表型芯片，代谢指纹图谱，表型特征

Abstract:

Abstract:［Objective］ We studied the phenotypic characterization of Phytophthora parasitica Dastur var．nicotianae．［Methods］ Phenotypic characterization of the pathogen was studied to provide information for disease management program by using BIOLOG phenotype MicroArray (PM)． Using PM plates 1 to 10，950 different phenotypic characterizations were tested.［Results］ P．parasitica was able to metabolize 74% of tested carbon sources，96% of nitrogen sources，100% of sulfur sources，and 98% of phosphorus sources． Most informative utilization patterns for carbon sources of P． parasitica were organic acids and carbohydrates，and for nitrogen were various amino acids． The pathogen presented 285 different nitrogen pathways． It had wide range adaptabilities in osmolytes with up to 1% sodium chloride，up to 3% potassium chloride，up to 5% sodium sulfate，up to 20% ethylene glycol，up to 2% sodium formate，up to 5% urea，and up to 2% sodium lactate． It also exhibited active metabolism under pH values between 3.5 and 10，with optimal pH of around 7.0．The pathogen showed both decarboxylase and deaminase activities in the presence of various amino acids．［Conclusion］These phenotypic characterizations of P．parasitica provided the theoretical basis for the next study of the pathogen in physiology and metabolism，and provided potential new way for tobacco black shank management.

Key words:Keywords: tobacco black shank，Biolog phenotype microarray，metabolic fingerprint，phenotypic characterization CLC number:Q933 Article ID:0001-6209(2015)10-1356-08