肌苷生产菌枯草芽孢杆菌ATCC 13952的全基因组测序及序列分析

Whole-genome sequencing and analysis of inosineproducing strain Bacillus subtilis ATCC 13952

摘要:

摘要:【目的】枯草芽孢杆菌ATCC 13952是一株肌苷工业生产菌株。为深入研究ATCC 13952菌株积累肌苷的分子机制以及为进一步分子育种研究提供序列背景信息，有必要解析ATCC 13952菌株的基因组序列信息。【方法】本研究采用高通量测序和Sanger测序相结合对ATCC 13952菌株进行全基因组测序，然后使用相关软件对测序数据进行基因组组装、基因预测与功能注释、GO/COG 聚类分析、共线性分析等。【结果】枯草芽孢杆菌ATCC 13952整个基因组大小为3876276 bp，GC含量为45.8%，序列已提交至GenBank 数据库，登录号为CP009748。比较基因组及嘌呤代谢相关基因分析结果显示:枯草芽孢杆菌ATCC 13952与其他几株芽孢杆菌具有较好的基因组共线性关系，嘌呤代谢相关基因编码的蛋白与标准菌株比较发生了一些缺失和突变。【结论】本研究首次报道了一株肌苷生产菌枯草芽孢杆菌ATCC 13952的全基因组序列，分析了基因组基本特征，初步探讨了该菌株积累肌苷的分子机制，为后续的进一步分子育种提供了理论基础。

Abstract:

Abstract:［Objective］Bacillus subtilis ATCC 13952 is an inosine-producing strain．In order to study the mechanisms of inosine accumulation and offer help for molecular breeding，it is necessary to uncover the genome sequence of ATCC 13952.［Methods］Whole-genome sequencing of ATCC 13952 is carried out by Solexa and Sanger sequencing．Genome assembly，gene prediction and functional annotation，GO/COG cluster analysis and synteny analysis are done using relevant software.［Results］The complete genomic information of Bacillus subtilis ATCC 13952 is contained on a single circular chromosome of 3876276 bp with an average GC content of 45.8%．The genome sequence is deposited in the GenBank under the accession number CP009748． Comparative genomic analysis shows that ATCC 13952 should have significant genomic synteny with other Bacillus subtilis strains．On the other hand，some point mutation and deletions occurred in purine metabolism-related genes between ATCC 13952 and the standard strain.［Conclusion］The results of this study will provide a theoretical basis for subsequent further molecular breeding.