胶州湾污染海域半知菌群体结构多样性时空动态变化及与生境的关系

Seasonal and spatial variation of Deuteromycetes population in polluted cost of Kiaochow Bay

摘要:

[目的] 揭示胶州湾典型污染海域半知菌群体结构多样性动态变化与生境的关系。[方法] 根据以往对近岸污染调查记录,分别确定胶州湾海泊河、李村河入海口为中度、重度污染采样站位,石老人潮间为轻度污染对照站位,利用PCR-DGGE指纹图谱方法,研究该生境半知菌群体结构多样性季节动态变化与污染的关系。[结果] 结果证明,污染指标除总氮含量外,其他各项指标含量在三站位的变化趋势为石老人潮间带最低,海泊河居中,李村河含量最高;与石老人站位相比,海泊河、李村河站位污染指标在四季变化较显著。首次证明在重金属和氮磷严重超标的海域,仍存在数量和种类较多的半知菌。PCR-DGGE指纹图谱结果显示,同一站位相似污染程度采样点之间DGGE指纹图谱比较一致,不同站位、不同季节之间指纹图谱存在明显的差异。优势条带割胶测序分析显示,3个站位存在大量的不可培养真菌,青霉属为半知菌优势种,重度污染的李村河入海口除半知菌外,存在较大量的阿米巴虫、腐霉等动物病原菌。[结论] 污染海域的半知菌群体结构与生境污染程度及季节具有较密切的联系。

关键词:污染海域;半知菌;PCR-DGGE;时空多样性

Abstract:

[Objective] To reveal the relationship between Deuteromycetes community and the environmental in Kiaochow Bay of the Yellow Sea. [Methods] Using recorded pollution survey, we used molecular methods to study seasonal and spatial variation of Deuteromycetes community diversity in different polluted waters of Kiaochow Bay of the Yellow Sea, China. [Results] Denaturing gradient gel electrophoresis fingerprints varied obviously among different sites of similar level of pollution. Moreover, sequence analysis of recovered dominant bands exhibited the existence of plenty of uncultivable fungi, among which Penicillium was the dominant genus. Furthermore, in heavily polluted estuary, there were abundant animal pathogens such as amoeba and Pythium as well as Deuteromycetes. These discoveries demonstrate that the Deuteromycetes community structure is closely related to marine environment, and are indicative of different level of marine contamination. [Conclusion] The relationship between Deuteromycetes community and different level of pollution and seasons varied were closely related.

Key words:polluted marine area;deuteromycetes;PCR-DGGE;temporal and spatial diversity