云南高原湖泊杞麓湖冬季可培养酵母菌多样性分析

Diversity of cultivable yeast in Qilu Lake in winter

摘要:

[目的] 探究云南杞麓湖酵母菌群落结构及其与环境因子的相互关系。[方法] 采用原位培养方法对杞麓湖14个水样进行酵母菌分离,应用26S rDNA D1/D2区域序列分析,并结合形态及生理生化指标将对分离获得的酵母菌进行鉴定,运用软件bio-dap和Canoco分析酵母菌类群的丰富度及其与环境因子间的相互关系。[结果] 从杞麓湖中分离得到321株酵母菌,鉴定为14个属27个种和1个潜在的新类群。Rhodosporidium kratochvilovae和出芽短梗霉(Aureobasidium pullulans)是优势种,分别为总菌株数的29.6%和16.8%。水体总磷含量是影响产色素红冬孢酵母属(Rhodosporidium)分布的重要环境因子,而pH为隐球酵母属(Cryptococcus)分布的一个重要选择条件。[结论] 杞麓湖酵母菌具有较为丰富的群落多样性。

Abstract:

[Objective] To investigate yeasts diversity in Qilu Lake and analyze the influence of environmental factors on yeast diversity. [Methods] Yeasts were isolated by in-situ cultivation and analyzed for the D1/D2 domain of large subunit (26S) ribosomal DNA and morphological characterization. We analyzed yeast species richness and species distribution in the Qilu Lake. [Results] In total 321 isolated yeasts were identified to 14 genera and 27 species. Rhodosporidium kratochvilovae and Aureobasidium pullulans were the dominating species in the lake, representing 29.6% and 16.8% of the total strains, respectively. Principal component analysis (PCA) showed that the total phosphorus was an important environmental factor affecting the distribution of Rhodosporidium and the pH affected Cryptococcus yeasts distribution. [Conclusion] There was a high diversity of yeasts community in the Qilu Lake.