消化乳杆菌泡菜分离株W369的鉴定及其益生功能

Identification of a new probiotic Lactobacillus alimentarius W369 from Chinese traditional pickles

摘要:

[目的] 鉴定从中国传统发酵泡菜分离的1株乳酸菌,并分析其潜在的益生功能。[方法] 通过形态观察、生理生化、16S rRNA基因序列分析等方法对分离得到的乳酸菌进行鉴定;评价该菌株的NaCl耐受能力、抗生素最小抑制浓度,以及益生功能。[结果] 该菌株鉴定为消化乳杆菌(Lactobacillus alimentarius) W369,在10%的NaCl浓度下仍可以存活;对常用抗生素具有不同程度的敏感性。益生功能分析表明,培养72 h后W369对亚硝酸钠的降解率为92.92%;其体外胆固醇脱除率达到31.80%;发酵液上清具有清除DPPH自由基(DPPH·)、超氧阴离子自由基(·O₂^-)和螯合亚铁离子(Fe²⁺)的作用,分别达到(88.02±1.48)%、(43.75±3.10)%和(29.99±2.34)%;完整细胞具有很强的还原能力[(244.52±0.92) μmol/mL半胱氨酸当量]、羟自由基(·OH)清除能力(77.17±0.58)%和抗脂质过氧化的能力(29.67±0.77)%;发酵上清液和无细胞提取物均具有谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(Mn-SOD)活性。[结论] 消化乳杆菌W369分离株具有亚硝酸盐降解能力、胆固醇清除能力和抗氧化活性等诸多益生功能,具有潜在的应用价值。

Abstract:

[Objective] A new lactic acid bacterium was isolated from Chinese traditional pickles and its potential probiotic properties were analyzed. [Methods] The strain was identified by morphological observation, physiological and biochemical tests and 16S rRNA gene comparison. Its probiotic properties were evaluated, including resistance to NaCl, the minimum inhibitory concentration of antibiotics, nitrite degradation ability and cholesterol removal ability. [Results] This strain was identified as Lactobacillus alimentarius W369 (No. CGMCC 7.180). L. alimentarius W369 could survive under the concentration of 10% NaCl and it was sensitive to some common antibiotics. By studying its probiotic properties, we found that its nitrite degradation rate reached 92.92% after 72 h and the cholesterol removal rate reached 31.80%. The fermented supernatant showed strong abilities of DPPH· and the superoxide anion radical scavenging capacity, as well as Fe2+ chelating ability. The clearance rate were (88.02±1.48)%, (43.75±3.10)% and (29.99±2.34)% respectively. Strain W369 also exhibited strong reducing capacity, hydroxyl radical scavenging action and lipid peroxidation inhibition capacity. Both the fermented supernatant and cell-free extracts exhibited glutathione peroxidase (GSH-Px) and superoxide dismutase (Mn-SOD) activities. [Conclusion] L. alimentariusW369 possessed many probiotic properties including nitrite degradation ability, cholesterol removal ability and antioxidant activity. It has many potential application values.