黑龙江马铃薯Y病毒P1基因的特点

Characteristics of P1 gene of potato virus Y in Heilongjiang

摘要:

[目的] 本研究通过对不同PVY分离物基因的测序及分析,从而了解PVY株系的多样性,进而对PVY病毒的分子检测及防治提供重要的资料和参考。[方法] 本研究针对黑龙江15个马铃薯Y病毒样品的P1基因进行克隆测序和进化树分析。[结果] 经比对分析,样品被分成两组,有10个样品的基因类型高度同源,且相对保守,是本地区的优势群组,无论是与国内其它地区样品比较还是与国外样品比较,其亲缘关系都有一定距离;而另一组中的5个样品的P1基因与本地优势组群有较大差异,且这5个样品间也有一定的差异,并与国内其它地区和国外一些样品的P1基因序列比较接近。通过比对GenBank中已上传的序列提供的PVY株系的信息,得知本次试验的P1基因与PVY^NTN-NW株系是相似的,且这15个样品与国内其他样品一样都是由PVY^N株系演变而来。[结论] 由P1基因分析表明,PVY受环境影响较大,黑龙江10个样品的PVY在长期的进化中产生了具有地方特点的变化,而后来的5个样品说明中国大部分PVY有可能是跟随国外品种资源的引进进入,同时PVY也随国内不同区域间资源交流和种薯调运而传播。

Abstract:

[Objective] Based on different potato virus Y isolates gene sequencing, we studied the diversity of potato virus Y strains, to provide information for molecular detection, prevention and control of the virus. [Methods] P1 gene of 15 samples of potato virus Y of Heilongjiang Province was cloned and then the sequences of genes were analyzed by using phylogenetic tree. [Results] Samples were divided into two groups. According to a comparative analysis, 10 samples have highly conservative and homologous genes. They are the dominant population in the research area and have certain genetic distance to other domestic samples and foreign samples. In another group, 5 samples differ significantly with local dominant population in term of P1 gene. These 5 samples also have some differences and their P1 genes are close to those of other domestic samples and foreign samples. By comparing PVY strain data provided by uploaded sequences in GenBank, it found that P1 gene of test samples is similar with PVY^NTN-NW strains. These 15 samples as well as other domestic samples are evolved from PVY^N strains. [Conclusions] The P1 gene analysis demonstrated that PVY is influenced by environment significantly and PVY of 10 samples in Heilongjiang develops local characteristics in the long-term evolution. The later 5 samples reflect that most PVY in China may be introduced by foreign cultivars. At the same time, PVY spreads through regional resource exchange and tuber transportation in China.