植物发酵液提取物对铜绿假单胞菌生物膜的作用

Effects of plant fermentation extract (PFE) on biofilms of Pseudomonas aeruginosa

摘要:

[目的] 探讨植物发酵液提取物(plant fermentation extract,PFE)对铜绿假单胞菌生物膜的抑制作用,为临床上铜绿假单胞菌感染相关疾病的治疗提供参考。[方法] 通过划线法分离临床标本中的铜绿假单胞菌并进行鉴定,通过报告菌株测定铜绿假单胞菌的毒力因子,采用试管法和激光共聚焦扫描显微镜测定生物膜的形成。[结果] 在分离出的16 株铜绿假单胞菌中,PFE对PA007菌株的作用效果最好,1%PFE显著降低PA007菌株生物膜、绿脓菌素和N-(3-oxododecanoyl)-HSL(3-oxo-C12-HSL)的产量(P<0.05)。同时,也显著降低LasA蛋白酶的活性以及持留菌存活率(P<0.05)。荧光定量PCR实验结果表明PFE能显著抑制lasI和pqsA基因的表达(P<0.05)。[结论] PFE具有抗铜绿假单胞菌感染能力,在临床上铜绿假单胞菌感染疾病的治疗中具有巨大的潜在价值。

Abstract:

[Objective] We studied the effects of a plant fermentation extract on destroying biofilms of P. aeruginosa, to provide basic information for treatment of P. aeruginosa related infection diseases. [Methods] Strains of P. aeruginosa in clinical specimens were isolated by streaking plate method and identified by PCR and sequencing. Virulence factors were examined using reporter strains, and biofilms were measured by test tube method and a confocal laser scanning microscopy. [Results] A total of 16 strains of P. aeruginosa were isolated from clinical specimens from a local hospital, among them PA007 strain showed a maximum response when treated with plant fermentation extract. It shows that 1% plant fermentation extract significantly reduced the production of biofilm, pyocyanine and 3-oxo-C12-HSL (P<0.05). Besides, 1% plant fermentation extract also deceased the bioactivity of LasA protease and survival rate of persisters (P<0.05). The qRT-PCR result indicated that the expressions of lasI and pqsA genes were also markedly inhibited at the presence of 1% plant fermentation extract (P<0.05). [Conclusion] The studied plant fermentation extract has anti-infection effect against some P. aeruginosa strains, suggesting a great potential to work as natural antibiotics.