雄烯二酮高产菌新金分枝杆菌MN4的全基因组测序及序列分析

Whole-genome sequencing and analysis of a adrostenedione yielding strains Mycobacterium neoaurum MN4

摘要:

[目的] 新金分枝杆菌（Mycobacterium neoaurum） MN4是1株经诱变育种获得的高产雄烯二酮，并且能够耐受高浓度底物植物甾醇的突变菌株。为深入研究MN4菌株耐底物的机制及雄烯二酮的生物合成途径，有必要解析MN4菌株的全基因组序列信息。[方法] 本研究采用高通量测序技术对MN4进行全基因组测序，然后使用相关软件对测序数据进行基因组装、基因预测与功能注释、COG聚类分析以及次级代谢产物合成基因簇预测等。[结果] 新金分枝杆菌MN4基因组装获得33个Contigs，整个基因组大小为5.39 Mb，GC含量为66.9%，编码4920个蛋白基因，序列提交至GenBank数据库，登录号为JXYZ00000000。[结论] 本研究首次报道了1株高产雄烯二酮菌株MN4的全基因组序列，分析了基因组的基本特征，初步解析了该菌株降解植物甾醇生产雄烯二酮的关键基因，将为MN4的功能基因组学研究及相关次级代谢产物的生物合成途径与异源表达研究提供基础。

Abstract:

[Objective] Mycobaterium neoaurum MN4 is a substrate-resistant mutant strain with high-yield androstenedione. In order to further study MN4 strain substrate-resistant mechanism and androstenedione biosynthetic pathway, it is necessary to decipher the MN4 strain genome.[Methods] The genome was sequenced using highthroughput sequencing technology, and analyzed using relevant software for genome assembly, gene prediction and functional annotation, COG cluster analysis and secondary metabolite biosynthesis gene clusters prediction.[Results] The whole genome is assembled into 33 contigs, and the genome size is 5.39 Mb, GC content of 66.9% with encoding 4920 protein genes. The genome sequence was deposited in the GenBank database under the accession number JXYZ00000000.[Conclusion] This study is the first report of androstenedione producing strain Mycobacterium neoaurum MN4 genome sequence, and provides a theoretical basis for further heterologous expression of secondary metabolites on Mycobacterium neoaurum MN4.