多杀性巴氏杆菌分子分型方法简述

Molecular typing methods for Pasteurella multocida-A review

摘要:

多杀性巴氏杆菌是一种能感染多种动物甚至是人的重要革兰氏阴性病原菌。目前临床上用于多杀性巴氏杆菌诊断的分型方法主要包括血清学分型方法和分子分型方法。其中血清学分型方法主要基于免疫学实验技术建立，操作过程繁琐，技术要求高，工作量大，不适用于临床上大规模快速开展多杀性巴氏杆菌流行病学调查的需要；而基于分子生物学手段建立的分子分型方法相对于传统的血清学分型方法而言具有快速、简单、灵敏、灵活等特点，特别是某些分子分型方法与传统的分型方法形成了较为精确的对应关系，因而在临床上得到了广泛的应用。目前适用于临床上开展多杀性巴氏杆菌分离鉴定的分子分型方法主要包括多重PCR方法及多位点序列分型法（MLST），其中多重PCR方法又包括基于荚膜编码区及脂多糖外核编码簇建立的PCR方法。本文将重点就这3种常用的多杀性巴氏杆菌分子分型方法进行综述，介绍其建立原理、实现手段以及各自的优缺点，为临床上开展多杀性巴氏杆菌的流行病学调查特别是分子流行病学调查提供参考。

关键词:多杀性巴氏杆菌;分子分型方法;多重PCR;多位点序列分型

Abstract:

Pasteurella multocida is an important gram-negative pathogenic bacterium that could infect wide ranges of animals. Humans could also be infected by P. multocida via animal bite or scratching. Current typing methods for P. multocida include serological typing methods and molecular typing methods. Of them, serological typing methods are based on immunological assays, which are too complicated for clinical bacteriological studies. However, the molecular methods including multiple PCRs and multilocus sequence typing (MLST) methods are more suitable for bacteriological studies of P. multocida in clinic, with their simple operation, high efficiency and accurate detection compared to the traditional serological typing methods, they are therefore widely used. In the current review, we briefly describe the molecular typing methods for P. multocida. Our aim is to provide a knowledge-foundation for clinical bacteriological investigation especially the molecular investigation for P. multocida.

Key words:Pasteurella multocida;molecular typing methods;multiple PCRs;multilocus sequence typing