牛源金黄色葡萄球菌FnBPA-A分子不同遗传多态性重组质粒的免疫生物学特性的比较

Immunological characteristic comparison of different genetic polymorphism recombinant of FnBPA-A of bovine Staphylococcus aureus strain

摘要:

[目的] 为了解FnBPA-A分子遗传多态性对新疆部分地区牛源金黄色葡萄球菌免疫生物学特性的影响。[方法] 对采自新疆不同地区的牛源金黄色葡萄球菌FnBPA-A氨基酸序列进行分析，构建了FnBPA-A 8个不同遗传多态性的真核重组质粒，分别免疫C57BL/6小鼠，收集免疫后的抗血清。对不同重组质粒免疫小鼠后免疫保护力进行比较分析。[结果] 进化树显示GS801、GS819、GS856属于同一分支，GW10-1、GW20-2、GY288、GY309为同一分支，GY278为一分支。免疫小鼠并进行攻击保护检测，GW20-2、GS801、GS819、GS856与GY288免疫组对小鼠的免疫保护率较高，GY278免疫组免疫保护率最低。[结论] FnBPA-A分子的遗传多态性可以影响免疫小鼠的免疫水平和攻毒保护力。

Abstract:

[Objective] To understand the impact of genetic polymorphism of FnBPA-A on the immune biological characteristics of Staphylococcus aureus.[Methods] Sequence of FnBPA-A of Staphylococcus aureus isolated from bovine in Xinjiang was analyzed and 8 different genetic polymorphism eukaryotic recombinants of FnBPA-A were constructed. C57BL/6 mice were immunized with these recombinant plasmids and mice sera were collected. Level of the immune protection of immunized mice was compared.[Results] GS801, GS819 and GS856 were on the same branch; GW10-1, GW20-2, GY288 and GY309 belong to the same branch; GY278 was on a different branch. For the challenge experiment, GW20-2, GS801, GS819, GS856 and GY288 showed better protection.[Conclusion] The genetic polymorphism of FnBPA-A could significantly affect the immune protection of immunized mice.