聚苹果酸生产菌出芽短梗霉CCTCC M2012223的全基因组测序及序列分析

Complete genome sequencing of polymalic acid-producing strain Aureobasidium pullulans CCTCC M2012223

摘要:

摘要：【目的】解析出芽短梗霉CCTCC M2012223的基因组序列信息，分析其代谢产物聚苹果酸、黑色素、普鲁兰多糖合成相关基因，为深入研究遗传多样性和代谢工程改造提供序列背景信息。【方法】使用Illumina HiSeq高通量测序平台对出芽短梗霉CCTCC M2012223菌株进行全基因组测序，并对测序数据进行序列拼接，基因预测与功能注释，COG/GO聚类分析，比较基因组学分析等。下载其他5株出芽短梗霉基因组序列，比较分析6株菌的种内同源基因、全基因组进化以及代谢产物合成相关基因。【结果】出芽短梗霉CCTCC M2012223基因组序列全长30756831 bp，GC含量47.49%，编码9452个基因。比较基因组分析表明出芽短梗霉CCTCC M2012223的基因组组装长度最长，6株菌的同源基因数达到7092个，普鲁兰多糖和聚苹果酸合成相关基因的蛋白序列有很高的保守性。出芽短梗霉CCTCC M2012223和Aureobasidium pullulans var. melanogenum亲缘关系最近，而这2株菌的黑色素合成相关基因的蛋白序列有一些插入和突变。【结论】本研究解析了出芽短梗霉CCTCC M2012223的基因组序列信息，获得黑色素、普鲁兰多糖和聚苹果酸合成相关基因，为后续的代谢机制解析和改造提供相关依据。

Abstract:

Abstract: [Objective] To explore the genome sequence of Aureobasidium pullulans CCTCC M2012223, analyze the key genes related to the biosynthesis of important metabolites, and provide genetic background for metabolic engineering. [Methods] Complete genome of A. pullulans CCTCC M2012223 was sequenced by Illumina HiSeq high throughput sequencing platform. Then, fragment assembly, gene prediction, functional annotation, and GO/COG cluster were analyzed in comparison with those of other five A. pullulans varieties. [Results] The complete genome sequence of A. pullulans CCTCC M2012223 was 30756831 bp with an average GC content of 47.49%, and 9452 genes were successfully predicted. Genome-wide analysis showed that A. pullulans CCTCC M2012223 had the biggest genome assembly size. Protein sequences involved in the pullulan and polymalic acid pathway were highly conservative in all of six A. pullulans varieties. Although both A. pullulans CCTCC M2012223 and A. pullulans var. melanogenum have a close affinity, some point mutation and inserts were occurred in protein sequences involved in melanin biosynthesis. [Conclusion] Genome information of A. pullulans CCTCC M2012223 was annotated and genes involved in melanin, pullulan and polymalic acid pathway were compared, which would provide a theoretical basis for genetic modification of metabolic pathway in A. pullulans.