东北稻田水体噬藻体psbA基因多样性

Genetic diversity of psbA of cyanophage from paddy floodwater in northeast China

摘要:

摘要：【目的】揭示东北稻田噬藻体psbA基因多样性，分析其系统进化地位，为噬藻体生态学研究提供数据支持。【方法】采用滤膜分离并浓缩噬体、PCR-克隆测序技术对我国东北稻田水体中噬藻体psbA基因进行调查。【结果】在东北稻田水体中共得到17条来自于噬藻体的psbA基因，经系统进化分析表明，我国东北稻田具有新的噬藻体的类群，与日本稻田生态系统中psbA基因类群相比，两地间噬藻体类群存在显著的差异，稻田水体中噬藻体psbA基因类群有别于海洋、湖泊类群。【结论】采用PCR-克隆测序技术以psbA基因为分子标记首次对我国东北稻田水体噬藻体类群进行调查，发现有新的噬藻体类群。

关键词:关键词：噬藻体，psbA基因，稻田水体

Abstract:

Abstract: [Objective] To provide scientific data for studying the ecology of cyanophage, we studied the genetic diversity of psbA of cyanophage from paddy floodwater in northeast China and its phylogenetic positions. [Methods] Membrane separation and concentration of cyanophage, PCR-cloning-sequencing were applied to study the diversity of psbA of cyanophage from paddy floodwater in northeast China. [Results] In total 17 psbA sequences of cyanophage were obtained. Novel cyanophages were found by phylogenetic analysis. Compared to those of Japanese paddy floodwater, marine and lakes, psbA gene assemblage of paddy floodwater in northeast China was significantly different. [Conclusion] This is the first report to study genetic diversity of cyanophage from paddy floodwater in northeast China with a molecular marker of psbA by PCR-cloning-sequencing. The novel psbA assembly of cyanophage was found in paddy floodwater in northeast China.

Key words:Keywords: cyanophage, psbA gene, paddy floodwater