桑椹肥大性菌核病病原菌生物学特性及流行性

Biological and epidemiological characteristics of the pathogen of hypertrophy sorosis scleroteniosis, Ciboria shiraiana

摘要:

[目的]对桑椹灾害性真菌病害——桑椹肥大性菌核病病原菌，即桑实杯盘菌（Ciboria shiraiana）的生物学特性进行研究，分析其流行性。[方法]采用人工接种、调查等研究方法，对C. shiraiana在无性生长阶段中菌丝侵染能力，菌核的休眠期，有性生长阶段中子囊孢子的结构、释放、数目以及萌发等进行研究，并对菌核萌发的物候期进行调查。[结果]C. shiraiana菌丝对桑雌花没有侵染能力；C. shiraiana菌核具有休眠期，低温处理6周以上的菌核才能萌发形成子囊盘；1个菌核可萌发1-15个子囊盘，直径为1.5 cm的子囊盘能产生高达（5.6-6.3）×10⁷个子囊孢子；C. shiraiana子囊孢子在酸性环境中的萌发率明显高于在中性和碱性环境中的萌发率；C. shiraiana菌核萌发形成子囊盘产生子囊孢子的物候期，从1月下旬开始到4月中旬结束，其中在3月中旬萌发子囊盘的数目达到最高值。[结论]桑椹肥大性菌核病属于典型的流行性侵染病，在果桑栽培上容易造成毁灭性危害，生产上必须高度重视该病的防控。

Abstract:

[Objective] We studied the biological and the epidemiological characteristics of the pathogen of hypertrophy sorosis scleroteniosis, which is a devastating fungal disease of mulberry. [Methods] We studied the asexual and sexual reproductive phase stages of C. shiraiana, including the infection ability of hyphal, dormancy of sclerotia, the structures, release, number and germination of ascospores from apothecia, as well as the phenology of sclerotial germination. [Results] In C. shiraiana, hyphae had no infection ability toward the female flowers of mulberry. Sclerotia of C. shiraiana must undergo cold treatment above 6 weeks, then the dormancy-breaking sclerotia could germinate to apothecia. One to fifteen apothecia were germinated from one sclerotium, and the number of ascospores in a 1.5 cm diameter apothecia could contain up to (5.6-6.3)×10⁷. Ascospore C. shiraiana had significantly higher germination rates in acid than in neutral and alkaline environments. From late January to middle April, sclerotia germinated to apothecia, and got the highest value in the middle of March. [Conclusion] C. shiraiana is a formidable pathogen to cause epidemic disease and damage in mulberry.