枸杞根际土壤真菌群落多样性的高通量测序

Fungal community diversity in rhizosphere soil of Lycium barbarum L. based on high-throughput sequencing

摘要:

[目的]为了解不同枸杞产区枸杞根际真菌多样性的差异，从枸杞根际土壤真菌群落的角度试图解释宁夏枸杞优良品质与其土壤微生物菌群的联系。[方法]采用MiSeq高通量测序方法，分别将我国4个不同地区枸杞根际土壤样品进行ITS区的高通量测序，并分析了物种组成和丰度、Alpha多样性、Beta多样性和菌群结构，测定不同产区枸杞主要有效成分，同时对枸杞品质和其土壤理化因子及枸杞根际真菌种群多样性的相关性做了分析。[结果]多糖含量和甜菜碱含量均是中宁枸杞高于兴仁和精河，格尔木含量最低；宁夏兴仁、宁夏中宁、新疆精河3个地区菌群结构类似，子囊菌门和结合菌门占总菌群的80%左右，而青海格尔木除了子囊菌门占58%外，壶菌门和新丽鞭毛菌门远远高于其他3个地区，比例占总菌群的近25%；4个地区枸杞根际土壤真菌菌群结构相似性依次为宁夏兴仁、新疆精河、宁夏中宁、青海格尔木。[结论]枸杞根际土壤样品真菌物种组成丰富，不同地区枸杞根际土壤真菌种群结构有一定差异；枸杞主要有效成分含量与枸杞根际土壤真菌种群结构具有一定相关性。

关键词:枸杞;根际土壤;真菌群落多样性;高通量测序;CCA分析

Abstract:

[Objective] To study wolfberry rhizosphere fungal diversity in different regions, we attempted to explain the correlation between Ningxia wolfberry quality and soil microbial flora from the perspective of rhizosphere fungal community.[Methods] We used the miseq high-throughput sequencing method to analyze the Internally Transcribed Spacer (ITS) region in the rhizosphere soil samples of Lycium barbarum L. in four different regions of China. We analyzed Alpha diversity, Beta diversity and fungal community structure, and determined the main active ingredients of Lycium barbarum L. in the four regions, and analyzed the correlation between the quality of Lycium barbarum L. and soil physical and chemical factors and the diversity of rhizosphere fungi.[Results] Polysaccharide and betaine of Lycium barbarum L. in Zhongning were higher than Jinghe's and Xingren's, and Golmud's was the lowest. Species diversity and structure analysis of soil fungi in the rhizosphere of Lycium barbarum L. showed that species diversity and structure were similar in Jinghe, Xingren and Zhongning, Ascomycetes and Zygomycota accounted for about 80% of the total species, but Golmud's was different from other regions, ascomycetes accounted for 58% and Chytridiomycota and Neocallimastigomycota were much higher than the other three regions, nearly 25% of the proportion of total fungi. Beta diversity analysis showed that the similarity of fungal flora structure in the rhizosphere soil of the Lycium barbarum L. in four regions was Xingren, Jinghe, Zhongning and Golmud.[Conclusion] The research showed that the composition of fungal species in rhizosphere soil of Lycium barbarum L. was abundant and there were some differences in the population structure of soil fungi in the rhizosphere soil of different regions. Some active ingredients of Lycium barbarum L. had a certain correlation with the population structure of soil fungi in the rhizosphere. So, microorganism boost the growth and quality of Lycium barbarum L..

Key words:Lycium barbarum L.;rhizosphere soil;fungal community diversity;high-throughput sequencing;canonical correspondence analysis