水环境中重金属铜对异育银鲫肠道微生物的影响

Effect of heavy metal Cu on Allogynogenetic Carassius auratus intestinal microorganism

摘要:

[目的]了解水环境中重金属铜对异育银鲫肠道微生物组成及多样性的影响。[方法]采用试剂盒提取异育银鲫肠道总DNA，然后对总DNA进行16S rRNA进行扩增，构建异育银鲫肠道微生物16S rRNA基因克隆文库，最后进行数据分析。[结果]厚壁菌门、变形菌门和拟杆菌门为异育银鲫肠道中主要的细菌类群，在不同浓度的重金属铜胁迫处理后，厚壁菌门的含量明显降低。稀释性曲线、Venn图和多样性指数分析结果表明，重金属铜胁迫处理后异育银鲫肠道微生物多样性明显降低。[结论]重金属铜会使异育银鲫肠道微生物组成及多样性降低。此结果为研究重金属污染对异育银鲫健康状况的影响及异育银鲫养殖过程中病害的诊断奠定基础。

关键词:异育银鲫;肠道微生物;重金属铜

Abstract:

[Objective] To study the effect of the heavy metal Cu on the bacterial composition and diversity in Allogynogenetic Carassius auratus intestinal.[Methods] Total DNA was extracted by DNA extraction kit. The 16S rRNA gene of intestinal microbial diversity was amplified by universal PCR primers. Then the clone library was built to analyze data.[Results] The main bacterial community in Allogynogenetic Carassius auratus intestinal contained Firmicutes, Proteobacteria and Bacteroidetes. At different concentrations of Cu, the contents of Firmicutes in Allogynogenetic Carassius auratus gut significantly decreased. The results of Rarefaction Curyiyuyiyuve, Venn and Diversity index showed that gut microbial diversity decreased obviously.[Conclusion] Cu caused the microbial diversity in Allogynogenetic Carassius auratus decreased. This result provides a basis for further studies on the health of Allogynogenetic Carassius auratus relevant to heavy metal Cu.

Key words:Allogynogenetic Carassius auratus;intestinal microflora;heavy metal copper