氨基酸脱氢酶的催化机理、分子改造及合成应用

Catalysis,engineering and application of amino acid dehydrogenases

摘要:

氨基酸脱氢酶催化可逆的氨基酸氧化脱氨和酮酸的不对称还原胺化反应，热力学上反应平衡倾向于生成氨基酸方向，从原子经济学和对环境影响的角度来看，是具有极大优势的氨基酸合成方法之一。本文将主要阐述近年来在α-氨基酸脱氢酶催化机理、分子改造和合成应用方面的研究进展。

关键词:L-氨基酸脱氢酶;D-氨基酸脱氢酶;催化机理;酶工程;合成应用

Abstract:

Amino acid dehydrogenases catalyze the reversible oxidative deamination of amino acids and reductive amination of keto acids, with thermodynamic equilibrium favoring the reductive amination direction. This is one of the most attractive methods for the synthesis of amino acids with high atomic economy and less environmental impact. This review addresses recent advances in studies on reaction mechanism, molecular modification and synthetic applications of amino acid dehydrogenases.

Key words:L-Amino acid dehydrogenase;D-Amino acid dehydrogenase;catalytic mechanism;enzyme engineering;synthetic application