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第二信使分子c-di-AMP调控细菌中钾离子转运的机制
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国家自然科学基金（31270105）；中央高校基本科研专科基金（2662015PY175）


Second messenger c-di-AMP regulates potassium ion transport in bacteria
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    摘要:

    钾离子（K+）是维持生命体存活的必需元素。原核生物进化出一系列K+转运系统，如Kdp系统﹑Ktr系统和Trk系统等，来维持胞内相对恒定的K+浓度。环二腺苷酸单磷酸（cyclic diadenosinemonophosphate，c-di-AMP）是新发现的第二信使分子，可以与K+转运系统中的KdpD、KtrA和TrkA结合。当胞内c-di-AMP浓度高时，c-di-AMP会与K+转运蛋白结合，降低其转运活性。c-di-AMP的靶标除蛋白质外，还有RNA元件，即c-di-AMP的核糖开关。高浓度的c-di-AMP与其核糖开关结合后，可抑制下游K+转运蛋白编码基因，如kdp、ktr和trk操纵子以及kup基因的转录，从而调控K+的转运。总之，胞内高浓度的c-di-AMP抑制细菌对K+的吸收。c-di-AMP调控K+转运机制的研究，不仅丰富了K+转运的调控方式，而且也扩大了c-di-AMP的调控范围，为细菌的利用与防治提供了新思路。

    Abstract:

    Potassium ion (K+) is a ubiquitous monovalent cation necessary for all living cells. To maintain homeostatic intracellular K+ concentration, most prokaryotes possess several unique K+ uptake systems to transport K+. A newly found second messenger-cyclic diadenosine monophosphate (c-di-AMP), plays an important role in regulating K+ transport by binding to several K+ transport-related proteins, such as KdpD, KtrA and TrkA. When the intracellular c-di-AMP concentration is high, c-di-AMP can bind its receptor or effector proteins to inhibit transporter activity. In addition, riboswitch could also be targeted by c-di-AMP to control the transcription of downstream K+ transporter genes, including ktr, trk and kdp operon and kup gene. High intracellular c-di-AMP concentration depresses bacterial K+ uptake. Therefore, understanding the mechanism of K+ transport inhibition by c-di-AMP not only enriches the regulation mode of the K+ transport, but also sparkle new ideas for the control and applications of bacteria.

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蔡霞,何进. 第二信使分子c-di-AMP调控细菌中钾离子转运的机制[J]. 微生物学报, 2017, 57(10): 1434-1442

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  • 收稿日期:2016-11-16
  • 最后修改日期:2017-01-06
  • 在线发布日期: 2017-09-29
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