基于iTRAQ技术对植物乳杆菌FS5-5的耐盐特性分析

Analysis of salt tolerance of Lactobacillus plantarum FS5-5 based on iTRAQ technology

摘要:

[目的]对大酱中耐盐性较好的植物乳杆菌进行蛋白质组学研究，为植物乳杆菌盐胁迫应激机制的研究提供实验数据。[方法]本项研究以筛选自东北传统农家大酱的耐盐性较好的植物乳杆菌FS5-5为研究对象，绘制了其在0%、6.0%、7.0%和8.0%（W/V） NaCl浓度下的生长曲线，并利用iTRAQ技术研究了其在0%、6.0%、7.0%和8.0%（W/V） NaCl浓度下的蛋白质表达情况。[结果]植物乳杆菌FS5-5在0%、6.0%、7.0%和8.0%（W/V） NaCl浓度下到达对数生长期中期的时间点分别为5、10、12和12 h；以差异倍数在1.2倍以上且P<0.05为筛选条件对6.0%、7.0%和8.0%（W/V） NaCl浓度下与0%进行差异蛋白质的筛选，共筛选出1271个差异蛋白质。这些差异蛋白质主要参与糖代谢、氨基酸代谢、脂肪酸代谢、核苷酸代谢、应激反应、转运、PTS系统和核糖体代谢等。[结论]植物乳杆菌在高盐浓度下生长与能量合成蛋白质、应激蛋白质以及相容性溶质转运蛋白质的表达上调有密切关系。

Abstract:

[Objective] Proteomic analysis of Lactobacillus plantarum to salt stress could provide a theoretical basis for salt-tolerant mechanism involved in lactic acid bacteria.[Methods] Lactobacillus plantarum FS5-5 was originally isolated from traditional home-made fermented soybean paste from Northeast China. Growth was observed under 0%, 6.0%, 7.0% and 8.0% (W/V) NaCl, and protein expression was analyzed using iTRAQ technology.[Results] We collected bacteria at 5, 10, 12 and 12 h of the mid-logarithmic growth phase for proteomic analysis. Based on iTRAQ results, a total of 1271 differently expressed proteins were identified under different salt stress. They were involved in carbohydrate metabolism, amino acid metabolism, lipid metabolism, nucleotide metabolism, stress proteins, transporters, phosphotransferase system, ribosome, and so on.[Conclusion] The ability of L. plantarum FS5-5 to grow in high concentration of NaCl is connected with energy synthesis proteins, stress proteins and compatible solutes transporters.