原核生物全基因组中16S rRNA基因的识别

Recognition of 16S rRNA genes in prokaryotic genomes

摘要:

[目的]识别原核生物全基因组中的16S rRNA基因。[方法]本文依据基因序列的GC碱基含量、碱基3-周期性和马尔可夫链3个方面的特性，构建了识别原核生物全基因组中16S rRNA基因的三层过滤模型。[结果]经检验，模型的特异性、敏感性和马修斯相关系数分别为99.58%、91.60%和91.49%。[结论]结果表明，本文所提出的方法可以高效、准确地识别出16S rRNA基因。

Abstract:

[Objective] We identified 16S rRNA genes in genomes of prokaryotes.[Methods] We constructed a 3-layer filtering model based on the three features of GC bases content of the gene sequences, 3-base periodicity and Markov chain to recognize the 16S rRNA genes from prokaryotic genomes.[Results] The specificity, sensitivity and Matthews correlation coefficients of the model were 99.58%, 91.60% and 91.49%, respectively.[Conclusion] The results showed that the 16S rRNA genes can be identified efficiently and accurately by using our model.