一株东方醋酸杆菌分离与其促进果蝇生长发育

Isolation of Acetobacter orientalis and their promotion of the growth and development of Drosophila melanogaster

摘要:

[目的]分离与鉴定黑腹果蝇体内醋酸杆菌，并研究其对宿主生长发育的促进作用。[方法]利用醋酸杆菌选择性培养基分离果蝇肠道醋酸杆菌；通过革兰氏染色和16S rRNA基因比对鉴定菌种；肠道定植实验验证共生关系；发育历期和生长速率实验检测其促进果蝇生长作用；免疫荧光染色技术检测肠道细胞增殖；RT-PCR法检测促生长的分子标志物和相关的信号通路。[结果]菌株为东方醋酸杆菌（Acetobacter orientalis），可以持续地定植在果蝇肠道及其培养基中，并且明显促进果蝇的生长。东方醋酸杆菌通过胰岛素信号通路增加肠分裂细胞的数量和促进蜕皮激素的分泌。[结论]东方醋酸杆菌是果蝇的一种共生菌，对果蝇肠道结构和机体发育具有重要的作用。

关键词:果蝇;东方醋酸杆菌;共生;生长发育

Abstract:

[Objective] This study was aimed to isolate and identify Acetobacter strains from Drosophila melanogaster, and to study their roles in promoting the growth of hosts.[Methods] Selective culture method was used to isolate Acetobacter; Gram-staining and 16S rRNA gene blast was used to identify strains. Intestinal colonization test was used to verify the commensalism. Developmental time and growth rate were used to evaluate the effect of the bacterial isolates on hosts' development. Immunofluorescence staining was used to check the mitosis in gut. RT-PCR was used to assess the molecular biomarker of development and development-associated cellular signaling.[Results] The strain was identified as Acetobacter orientalis and it colonized the gut of Drosophila and fly medium, and significantly promoted the growth of the host. Furthermore, Acetobacter orientalis enhanced the number of mitotic cells in gut, and promoted the secretion of growth hormone of hosts by regulating the insulin signal pathway.[Conclusion] Acetobacter orientalis was one of commensal bacteria of Drosophila, and maintained the structure of intestine and promote the growth of hosts.

Key words:Drosophila;Acetobacter orientalis;commensalism;development