嗜麦芽窄食单胞菌OUC_Est10全基因组测序及脂类水解酶多样性分析

Complete genome sequencing and diversity analysis of lipolytic enzymes in Stenotrophomonas maltophilia OUC_Est10

摘要:

[目的]本研究的目的是研究嗜麦芽窄食单胞菌OUC_Est10中脂类水解酶的多样性。[方法]使用离子交换层析、全基因组测序和异源表达三种方法研究嗜麦芽窄食单胞菌OUC_Est10中脂类水解酶的多样性。[结果]离子交换层析结果显示嗜麦芽窄食单胞菌OUC_Est10可以分泌多种脂类水解酶。通过全基因组测序，我们给出了该菌的全基因组序列，该基因组大小为4668743 bp，GC含量为66.25%。通过详细的基因组序列分析，我们从该基因组中找到33个可能具有脂类水解酶活性的假定基因。通过异源表达OUC_Est10中的5个假定脂类水解酶基因，来研究其催化特性的多样性，结果显示这些脂类水解酶具有不同的催化特性。[结论]我们证明了嗜麦芽窄食单胞菌OUC_Est10拥有多样的脂类水解酶，这暗示了它在不同领域中的应用潜力。

关键词:嗜麦芽窄食单胞菌;分离;脂类水解酶;全基因组测序;催化特性

Abstract:

[Objective] The aim of this study was to study the diversity of lipolytic enzymes in Stenotrophomonas maltophilia OUC_Est10.[Methods] Ion exchange chromatography, genome sequencing and heterologous expression were used to study the diversity of lipolytic enzymes in Stenotrophomonas maltophilia OUC_Est10.[Results] Stenotrophomonas maltophilia OUC_Est10 could secret a wide range of lipolytic enzymes (lipases and esterases) as revealed by ion exchange chromatography. The complete genome is of 4668743 bp in length, with an average GC content of 66.25%. Genome annotation indicated the presence of 33 candidate genes whose products possess the predicted lipolytic enzyme activities. Analysis of catalytic features was carried out by expressing five putative lipolytic enzyme genes, and lipolytic enzymes in OUC_Est10 had different catalytic properties.[Conclusion] We proved that Stenotrophomonas maltophilia OUC_Est10 was a good candidate to produce diverse lipolytic enzymes, with potential applications in various fields.

Key words:Stenotrophomonas maltophilia;separation;lipolytic enzyme;complete genome sequence;catalytic properties