2025年5月15日 周四
首页 > 过刊浏览>2018年第58卷第2期 >238-246. DOI:10.13343/j.cnki.wsxb.20170101
PDF HTML阅读 XML下载 导出引用 引用提醒
luxS基因缺失对禽致病性大肠杆菌O1、O2和O78三种血清型分离株的生物学特性影响
作者:
基金项目:

国家自然科学基金（31370045，31372402，31572546）；上海市科技兴农重点攻关项目[沪农科攻字（2015）第1-9号]


Inactive luxS influence on biological characteristics of O1, O2 and O78 serotypes isolates in avian pathogenicity Escherichia coli
Author:
  • 摘要
    • | |
  • 访问统计
    • |
  • 参考文献 [26]
    • |
  • 相似文献 [20]
    • |
  • 引证文献
    • | |
  • 文章评论
    摘要:

    [目的]LuxS/AI-2型密度群体感应系统产生的自诱导信号分子AI-2（AI-2的产生需要luxS基因编码的LuxS蛋白参与）参与对细菌众多生理功能的调控。探讨luxS对不同血清型禽致病性大肠杆菌（Avian Pathogenicity Escherichia coli，APEC）生物学特性的影响。[方法]本研究以APEC优势血清型APECO1（O1血清型）、DE17（O2血清型）、E940（O78血清型）及其相应luxS缺失株为研究对象，对野生株和缺失株的生长特性、生物被膜形成、rdar （red，dry and rough）形态、运动性和耐药性等特性进行分析。[结果]luxS基因的缺失不影响APEC生长特性，但导致APEC不能产生AI-2；此外，luxS基因的缺失显著降低APECO1和E940的生物被膜形成（P<0.05），而DE17的生物被膜形成无显著变化。对各菌株的rdar形态和运动性检测结果表明，luxS基因的缺失改变了APECO1的rdar形态，对DE17和E940并无影响；显著降低了APECO1和DE17运动能力，对E940并无影响。荧光定量PCR检测结果表明，luxS基因的缺失显著降低APECO1、DE17和E940与细菌运动性相关的鞭毛基因fliG和fliI的转录水平（P<0.05）。此外，对各菌株的耐药性检测结果表明，luxS基因缺失导致APECO1对头孢吡肟和丁胺卡那由耐药变为高敏，同时对氯霉素与E940相同由高敏变为耐药，但对DE17的耐药性无显著改变。[结论]luxS对APEC的生物学特性具有重要的调控作用，且这种调控具有菌株特异性。

    Abstract:

    [Objective] LuxS/AI-2-type quorum sensing participates in regulation of bacterial biological properties by AI-2, which is synthesized by the enzymes LuxS (encoded by luxS). The aim of this study is to elucidate luxS effects on biological characteristics of different serotypes in Avian Pathogenic Escherichia coli (APEC).[Methods] The biological characteristics of 3 APEC strains, including APECO1 (serotype O1), DE17 (serotype O2) and E940 (serotype O78) and their luxS mutant strains were analysed by growth curve, biofilm-producing, rdar morphology, bacterial motility and sensitivity to antibiotics.[Results] luxS knockout, eliminating AI-2 formation, had no influence on bacterial growth, but significantly decreased biofilm-producing in E940 and APECO1 (P<0.05), without effect on DE17. Results of rdar morphology indicate that the APECO1 strain showed remarkable change in rdar morphology compared with luxS mutant, but no change in DE17 and E940. Moreover, deletion of luxS lead to decreasing the motility of APECO1 and DE17, but no influence on E940 strain. The transcriptional levels of fliG and fliI were significantly decreased in APECO1, DE17 and E940 (P<0.05). Furthermore, results of drug-resistance indicate that luxS inactivation caused APECO1 resistant to cefepime, amikacin, and sensitive to chloramphenicol. luxS inactivation also caused E940 strain sensitive to chloramphenicol, but no significant effect on drug-resistance of DE17 strain.[Conclusion] LuxS plays a significant role in regulation of biological properties in APEC.

    参考文献
    [1] Dho-Moulin M, Fairbrother JM. Avian pathogenic Escherichia coli (APEC). Veterinary Research, 1999, 30(2/3):299-316.
    [2] Stathopoulos C, Provence DL, Curtiss Ⅲ R. Characterization of the avian pathogenic Escherichia coli hemagglutinin Tsh, a member of the immunoglobulin a protease-type family of autotransporters. Infection and Immunity, 1999, 67(2):772-781.
    [3] Dou XH, Gong JS, Han XA, Xu M, Shen HY, Zhang D, Zhuang LL, Liu JS, Zou JM. Characterization of avian pathogenic Escherichia coli isolated in eastern China. Gene, 2016, 576(1):244-248.
    [4] Guabiraba R, Schouler C. Avian colibacillosis:still many black holes. FEMS Microbiology Letters, 2015, 362(15):fnv118.
    [5] Landman WJM, van Eck JHH. The incidence and economic impact of the Escherichia coli peritonitis syndrome in Dutch poultry farming. Avian Pathology, 2015, 44(5):370-378.
    [6] Pereira CS, Thompson JA, Xavier KB. AI-2-mediated signalling in bacteria. FEMS Microbiology Reviews, 2013, 37(2):156-181.
    [7] Xavier KB, Bassler BL. LuxS quorum sensing:more than just a numbers game. Current Opinion in Microbiology, 2003, 6(2):191-197.
    [8] Zhu JG, Dizin E, Hu XB, Wavreille AS, Park J, Pei DH. S-Ribosylhomocysteinase (LuxS) is a mononuclear iron protein. Biochemistry, 2003, 42(16):4717-4726.
    [9] Bai H, Han XG, Liu L, Shan XQ, Song J, Liu R, Dong HL, Liu HW, Ding C, Yu SQ. The regulation of autoinducer-2 in avian pathogenic Escherichia coli. Scientia Agricultura Sinica, 2012, 45(24):5110-5116. (in Chinese)白灏, 韩先干, 刘蕾, 单雪芹, 宋军, 刘瑞, 董洪亮, 刘海文, 丁铲, 于圣青. 信号分子AI-2对禽致病性大肠杆菌的调控作用. 中国农业科学, 2012, 45(24):5110-5116.
    [10] Han XG, Bai H, Liu L, Chen WJ, Ding C, Hu QH, Qi KZ, Yu SQ. Cloning and expression of luxS and pfs and in vitro biosynthesis autoinducer 2 of avian pathogenic Escherichia coli from Anhui Province. Acta Microbiologica Sinica, 2012, 52(9):1167-1172. (in Chinese)韩先干, 白灏, 刘蕾, 陈文静, 丁铲, 胡青海, 祁克宗, 于圣青. 禽致病性大肠杆菌安徽分离株luxS和pfs基因的克隆、表达与细胞外合成AI-2活性检测. 微生物学报, 2012, 52(9):1167-1172.
    [11] Han XG, Bai H, Liu L, Dong HL, Liu R, Song J, Ding C, Qi KZ, Liu HW, Yu SQ. The luxS gene functions in the pathogenesis of avian pathogenic Escherichia coli. Microbial Pathogenesis, 2013, 55:21-27.
    [12] Han XG, Lu CP. Biological activity and identification of a peptide inhibitor of LuxS from Streptococcus suis serotype 2. FEMS Microbiology Letters, 2009, 294(1):16-23.
    [13] Han XG, Lu CP. Detection of autoinducer-2 and analysis of the profile of luxS and pfs transcription in Streptococcus suis serotype 2. Current Microbiology, 2009, 58(2):146-152.
    [14] Chen WJ, Han XG, He L, Hu QH, Yu SQ. Characterization of duck pathogenic Escherichia coli. Chinese Journal of Animal Infectious Diseases, 2010, 18(2):34-40. (in Chinese)陈文静, 韩先干, 何亮, 胡青海, 于圣青. 鸭致病性大肠杆菌的分离鉴定及其生物学特性分析. 中国动物传染病学报, 2010, 18(2):34-40.
    [15] Zhang YX, Han XG, Zuo JK, Gong JS, Han Y, Fan GB, Wang SH, Tian MX, Ding C, Qi KZ, Yu SQ. Distribution of lipopolysaccharide core type in avian pathogenic Escherichia coli and its correlation with virulence gene. Microbiology China, 2015, 42(8):1619-1625. (in Chinese)张宇曦, 韩先干, 左佳坤, 龚建森, 韩月, 范国博, 王少辉, 田明星, 丁铲, 祁克宗, 于圣青. 禽致病性大肠杆菌脂多糖核心型分布与毒力基因的相关性分析. 微生物学通报, 2015, 42(8):1619-1625.
    [16] Reisner A, Krogfelt KA, Klein BM, Zechner EL, Molin S. In vitro biofilm formation of commensal and pathogenic Escherichia coli strains:impact of environmental and genetic factors. Journal of Bacteriology, 2006, 188(10):3572-3581.
    [17] Römling U. Characterization of the rdar morphotype, a multicellular behaviour in Enterobacteriaceae. Cellular and Molecular Life Sciences CMLS, 2005, 62(11):1234-1246.
    [18] Ling H, Kang A, Tan MH, Qi XB, Chang MW. The absence of the luxS gene increases swimming motility and flagella synthesis in Escherichia coli K12. Biochemical and Biophysical Research Communications, 2010, 401(4):521-526.
    [19] Ni B, Ghosh B, Paldy FS, Colin R, Heimerl T, Sourjik V. Evolutionary remodeling of bacterial motility checkpoint control. Cell Reports, 2017, 18(4):866-877.
    [20] Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative CT method. Nature Protocols, 2008, 3(6):1101-1108.
    [21] da Silveira WD, Ferreira A, Brocchi M, de Hollanda LM, de Castro AFP, Yamada AT, Lancellotti M. Biological characteristics and pathogenicity of avian Escherichia coli strains. Veterinary Microbiology, 2002, 85(1):47-53.
    [22] Paixão AC, Ferreira AC, Fontes M, Themudo P, Albuquerque T, Soares MC, Fevereiro M, Martins L, de Sá MIC. Detection of virulence-associated genes in pathogenic and commensal avian Escherichia coli isolates. Poultry Science, 2016, 95(7):1646-1652.
    [23] Redanz S, Standar K, Podbielski A, Kreikemeyer B. Heterologous expression of sahH reveals that biofilm formation is autoinducer-2-independent in Streptococcus sanguinis but is associated with an intact activated methionine cycle. Journal of Biological Chemistry, 2012, 287(43):36111-36122.
    [24] Weiss-Muszkat M, Shakh D, Zhou YZ, Pinto R, Belausov E, Chapman MR, Sela S. Biofilm formation by and multicellular behavior of Escherichia coli O55:H7, an atypical enteropathogenic strain. Applied and Environmental Microbiology, 2010, 76(5):1545-1554.
    [25] Nickzad A, Lépine F, Déziel E. Quorum sensing controls swarming motility of Burkholderia glumae through regulation of rhamnolipids. PLoS One, 2015, 10(6):e0128509.
    [26] Chait R, Craney A, Kishony R. Antibiotic interactions that select against resistance. Nature, 2007, 446(7136):668-671.
    引证文献
引用本文

吕小龙,胡剑刚,陈兆国,荆雅玮,米荣升,黄燕,王少辉,韩先干,祁克宗. luxS基因缺失对禽致病性大肠杆菌O1、O2和O78三种血清型分离株的生物学特性影响[J]. 微生物学报, 2018, 58(2): 238-246

复制
相关视频

分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2017-03-06
  • 最后修改日期:2017-05-04
  • 在线发布日期: 2018-01-26
文章二维码

科微学术

微生物学报

您是本站第 5928304 访问者

通信地址：北京市朝阳区北辰西路1号院3号 中国科学院微生物研究所   邮编：100101

电话：010-64807516    E-mail：actamicro@im.ac.cn

版权所有：微生物学报 ® 2025 版权所有