光电子和光生空穴对粪产碱杆菌代谢产物的影响

Effects of photoelectron and photogenerated hole on the metabolites of Alcaligenes faecalis

摘要:

[目的]探讨光催化下纳米TiN对粪产碱杆菌代谢情况的影响。[方法]我们通过分别添加空穴捕获剂及电子捕获剂，使用三维荧光光谱分析比较光生空穴和光电子对粪产碱杆菌（Alcaligenes faecalis）生长代谢的不同作用。[结果]光照条件下，空穴捕获剂组明显生成了较多的类腐殖质类物质，且比其他实验组有更强的NADH的荧光峰出现，峰强度是其他实验组的4到5倍。黑暗条件下，各实验组之间的代谢产物无明显变化。光照条件下的电子捕获剂组比黑暗条件下有更强的类蛋白质类荧光峰。[结论]本文首次报道光电子会促进粪产碱杆菌产生腐殖质类物质，且会产生更多的能量。光生空穴会促进粪产碱杆菌产生蛋白质类物质。

Abstract:

[Objective] To explore the effect of photoelectron and photogenerated hole on the metabolites of Alcaligenes faecalis. [Methods] We compared different effect of photoelectron and photogenerated hole on metabolism of Alcaligenes faecalis through the Three-dimensional fluorescence spectroscopy by adding the hole scavenger and electron capturer. [Results] Under light, the hole scavenger group obviously produced 4 to 5 times higher humus-like substances and intensity of NADH fluorescence peaks than the electron capturer group, while the Three-dimensional fluorescence spectra did not show visible change among the experimental sets under dark. In contrast, the electron capturer group under light shows stronger fluorescence peak than under dark. [Conclusion] This is the first report that the photoelectron will promote the Alcaligenes faecalis to produce humus substances and more energy. The photogenerated hole will promote the Alcaligenes faecalis to produce protein.